Figure 1 BG-12 treatment reduced total circulating B cells and had variable effects on memory B cells BG-12 treatment reduced total circulating B cells.

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Figure 1 BG-12 treatment reduced total circulating B cells and had variable effects on memory B cells BG-12 treatment reduced total circulating B cells and had variable effects on memory B cells Peripheral blood mononuclear cells (PBMC) isolated from patients with relapsing-remitting multiple sclerosis (RRMS) before and after BG-12 treatment and healthy controls were stained for B-cell markers. (A) Representative forward scatter, side scatter, and anti-CD19 staining from a single patient (RRMS-8) shows standardized gating for lymphocytes, single cells, and total CD19+ B cells that was used for all of the samples. (B) Comparison of %CD19+ B cells for healthy controls and patients with RRMS before treatment. Each dot represents an individual sample and lines are the median interquartile range for each group. The exact p value shown below the title was calculated using Mann-Whitney test. (C) Total B-cell numbers/mL of blood for the patients with RRMS were calculated before and after 4–6 months of treatment by multiplying the %CD19+ cells by the lymphocyte counts reported by the clinical pathology laboratory (table 1). Lines connect individual patients. Exact p value for paired patient data was determined using Wilcoxon matched-pairs signed-rank test. (D) Representative histograms from patient RRMS-8 show gating used to define CD27+ memory B cells. (E) Percentages of CD27+ memory B cells in patients with RRMS before treatment compared with controls. Each dot represents data from one individual and lines are the median and interquartile range for each group. The p value was calculated using Mann-Whitney test. (F) Longitudinal %CD27+ memory B-cell data for individual patients with RRMS (n = 8) before, after 4–6 months, and after more than 1 year of treatment with BG-12. Steven K. Lundy et al. Neurol Neuroimmunol Neuroinflamm 2016;3:e211 © 2016 American Academy of Neurology