FACT facilitates alternative splicing of RNA transcripts.

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FACT facilitates alternative splicing of RNA transcripts. FACT facilitates alternative splicing of RNA transcripts. (A) Volcano plot of depleted/enriched proteins at H3K4me3 following Ssrp1 depletion. (B) Bar plots representing the number of included/skipped exons categorized by their gene expression status (red: “down-regulated,” cyan: “unchanged,” and blue: “up-regulated”). In total, we have identified 149 included and 207 skipped exon events in the Ssrp1 KD group. (C) Graphical representation of an intronic retention event (Men1) in the KD group. Also, analysis of intron inclusion events or isoform switches after FACT depletion. Unspliced transcript percentage was measured according to band intensity. (D) Gene ontology analysis of transcripts (FACT-bound) that display alternative exon usage between the two conditions. Constantine Mylonas, and Peter Tessarz LSA 2018;1:e201800085 © 2018 Tessarz and Mylonas