Radio-HPLC analysis of the biosynthetic product (after chemical N-benzoylation; Rt = 39.6 ± 0.1 min) generated by the recombinant O-phenylpropanoyltransferase.

Slides:

Advertisements

Similar presentations

1 主讲：郑萍高级工程师，执业药师化学化工学院 2 液相色谱分析是在经典的液体柱色谱基础上，引入了气相色谱的理论；在技术上采用了高压泵、高效固定相和高灵敏度检测器，实现了分析速度快、分离效率高和操作自动化，这种柱色谱技术被称做高效液相色谱法。

Advertisements

Figure S3: LC-MS elution profiles of 84 benzoyl anthranilates produced by the recombinant 4CL5-HCBT yeast strain. ESI-MS spectra were obtained after LC-TOF.

The stimulatory effect of Taxol, epoB, and Disco on tubulin polymerization correlates well with their inhibitory effect on photolabeling of β-tubulin.

The secreted form of ORF2 (ORF2S) is glycosylated and dimerized.

ФОНД ЗА РАЗВОЈ РЕПУБЛИКЕ СРБИЈЕ

(A) Schematic illustration of the experimental design for the analysis of the RNase activity of MARF1 in oocytes by mutating the conserved D272 residue.

O. nubilalis damage and insecticide usage in Mid-Atlantic vegetable crops (1980–2017) and their relationship with Bt adoption. O. nubilalis damage and.

Posttranslational regulation of ETV5 and c-JUN in neurons.

F - Ratio Table Degrees of Freedom for the Factor

Does the effect of staying in school until age 16 depend on the BMI PGS? Bars show 2SLS point estimates of effect of staying in school until age 16 on.

Schematic representation of possible effects of different factors on systemic resilience. Schematic representation of possible effects of different factors.

The evolution of WCI for biological specificity makes organisms more evolvable. The evolution of WCI for biological specificity makes organisms more evolvable.

DHEA(S) and Allo induced the phosphorylation of PKC in serum-deprived PC12 cells. DHEA(S) and Allo induced the phosphorylation of PKC in serum-deprived.

(A) Schematic diagram of TGC

Bower building. Bower building. (A) Characteristic behavioral patterns associated with pit digging (Upper) and castle bower building (Lower). (B) Average.

Pretreatment of VSV-GPΔ to form VSV-GP19K rescues infection in the α5β1-integrin-negative CHO cells. Pretreatment of VSV-GPΔ to form VSV-GP19K rescues.

Fundamental epidemiological indicators.

DHEA(S) and Allo induced the expression of the antiapoptotic Bcl-2 proteins in serum-deprived PC12 cells. DHEA(S) and Allo induced the expression of the.

Variance priming for spatially nonoverlapping prime–target pairs.

Dark blue E. coli had lower growth rate and yield in monoculture but grew faster in coculture with S. enterica. Dark blue E. coli had lower growth rate.

Social activity of humans and bots over time.

The repeat domain of Tau cross-links to α-tubulin.

WCI evolve as organisms become more complex.

Estimation of R(t). Estimation of R(t). (A) Daily R(t) as inferred from the daily incidence of new infections for one stochastic model realization. Tg.

Detection of CD38+IgG1+ memory B cells adjacent to contracted GCs

Proposed reaction sequence initiating HMBPP reduction as it was derived from crystallographic data. Proposed reaction sequence initiating HMBPP reduction.

Confocal microscope images of Reelin mRNA detected by in situ hybridization using a biotinylated oligoprobe (base pairs 1264–1311) visualized with fluorescein.

HPLC tracings of resveratrol (RV) and its major sulfate conjugates M4/M5 in the plasma from three subjects. HPLC tracings of resveratrol (RV) and its major.

H3K4me3 pattern association with functionally relevant genes and changes over time. H3K4me3 pattern association with functionally relevant genes and changes.

HPLC-VIS chromatograms of extracts of authentic mirtocyan (A) and of plasma (B), urine (C), or colorectal tumor tissue (D) from patients who received 1.4.

Variance priming. Variance priming. (A) Mean RTs for both levels of prime variance (x axis; high or low) and both levels of target variance (lines; high.

Simulations capture the experimental force profiles for mutant I27 domains. Simulations capture the experimental force profiles for mutant I27 domains.

Structures of natural cytotoxicity receptors.

Mitofilin cluster distribution is denser in the perinuclear mitochondria. Mitofilin cluster distribution is denser in the perinuclear mitochondria. (A)

'III \-\- I ', I ,, - -

Volume 19, Issue 2, Pages (February 2012)

Retention times for identification of coproporphyrin.

MG132 allows extensive RT of HIV-1 cDNA despite exogenous rhTRIM5α.

Distinct TF motifs are associated with decreased or increased H3K27 acetylation peaks in hypothyroidism. Distinct TF motifs are associated with decreased.

Expression of NCoR1∆ID in PAX8−/− mice does not reverse repression of T3-positive target genes. qPCR on the indicated pituitary (A) and neuronal (B) genes.

In vivo BrdUrd incorporation kinetics of KTSL−/lo and KTSL− cells.

Oligosaccharide profile of VexL reaction products.

NMR characterization of the ADPr-binding C(M/P)Y and CYR motifs.

(A) Communication fluxes PS(T|r′) conditional to the rank r′ for dataset D3. (A) Communication fluxes PS(T|r′) conditional to the rank r′ for dataset D3.

Coumarin formation in novobiocin biosynthesis: β-hydroxylation of the aminoacyl enzyme tyrosyl-S-NovH by a cytochrome P450 NovI Huawei Chen, Christopher.

Catherine M. Pound et al. Hospital Pediatrics 2017;7:

IL-6Rα protein is rapidly synthesized by activated human PMNs

Sarcomere length-dependence of the M3 reflection at rest (A) and at the plateau of an isometric tetanus (B). Sarcomere length-dependence of the M3 reflection.

AKAP15 coimmunoprecipitates with CaV1

Separation by HPLC of lipopeptide adducts from the tryptic digest of involucrin. Separation by HPLC of lipopeptide adducts from the tryptic digest of involucrin.

Excision of N7-methylguanine by AlkZ.

(Upper) Blind-to-controls generalization performance: prediction accuracies observed when the classifier was trained on the auditory trials from the blind.

Peptide release is not impacted by phosphorothioate modification at interface of the P-site and A-site codons. Peptide release is not impacted by phosphorothioate.

Identification of the AlkZ active site.

Clustering of residues for which PREs unaccounted for by the main state were measured. Clustering of residues for which PREs unaccounted for by the main.

Quadratic model fitting to foveal measurements.

Nonsense and missense amino acid mutation analysis.

Schematic representation of our random walk model with environmental state-dependent dynamics. Schematic representation of our random walk model with environmental.

Multiple sequence alignment of STAT6 and other STAT proteins produced by ClusterW and ESpript (espript.ibcp.fr/ESPript/ESPript/). Multiple sequence alignment.

Quantitative analysis of CR evolution through conditioning sessions for the four experimental groups. Quantitative analysis of CR evolution through conditioning.

Snapshots of equilibrated structures of PrPC.

Example of the spontaneous plastic event exhibiting a concatenation of a series of Eshelby quadrupoles resulting in a correlated displacement field with.

Oscillatory inertial focusing with diffusion limitation.

Mediation model of study 4.

Steep decline of heterozygosity (based on 100-bp windows), Tajima’s D values on a chromosomal arm 2R region (8,700,000–8,820,000) in D. simulans (A), and.

Correlation between Ti→jS(r) and Ti→jM(r) for pair of locations (gray dots) separated by a distance of (A) r=1 km, (B) r=5 km, (C) r=20 km, (D) r=50 km,

ATR produced inside rhodopsin by light bleaching can exchange with an equimolar amount of exogenously added, radioactive [3H]ATR. ATR produced inside rhodopsin.

(a) Pattern of UWHBs, represented as green segments joining paired α-carbons, for human Hb β-subunit. (a) Pattern of UWHBs, represented as green segments.

HPLC chromatograms using mobile phase containing CTAB and OSA showing complete separation of: A, phthaloylglutamine and phthaloylisoglutamine authentic.

Presentation transcript:

Radio-HPLC analysis of the biosynthetic product (after chemical N-benzoylation; Rt = 39.6 ± 0.1 min) generated by the recombinant O-phenylpropanoyltransferase by using [13-3H]baccatin III and β-phenylalanoyl-CoA as cosubstrates. Radio-HPLC analysis of the biosynthetic product (after chemical N-benzoylation; Rt = 39.6 ± 0.1 min) generated by the recombinant O-phenylpropanoyltransferase by using [13-3H]baccatin III and β-phenylalanoyl-CoA as cosubstrates. The upper trace shows the radioactivity profile (in millivolts) and the lower trace shows the absorbance profile (A254) of authentic (3′RS)-2′-deoxytaxol. The radioactivity peak at 21.3 min is residual substrate. Kevin Walker et al. PNAS 2002;99:20:12715-12720 ©2002 by National Academy of Sciences