Comparison of developmental endothelial locus-1 angiogenic factor with vascular endothelial growth factor in a porcine model of cardiac ischemia Murray.

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Date of download: 7/3/2016 Copyright © The American College of Cardiology. All rights reserved. From: Cotransfection of Vascular Endothelial Growth Factor-A.

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Comparison of developmental endothelial locus-1 angiogenic factor with vascular endothelial growth factor in a porcine model of cardiac ischemia Murray H Kown, MD, Takeshi Suzuki, MD, Mark L Koransky, MD, Kalyani Penta, PhD, Gordon Sakamoto, MD, Christina L Jahncke, Andrew J Carter, DO, Thomas Quertermous, MD, Robert C Robbins, MD The Annals of Thoracic Surgery Volume 76, Issue 4, Pages 1246-1251 (October 2003) DOI: 10.1016/S0003-4975(03)00721-5

Fig 1 Coronary angiography of a representative heart 3 weeks after implantation of a copper stent. Note total occlusion of the left circumflex artery. (LAD = left anterior descending coronary artery; LCX = left circumflex artery.) The Annals of Thoracic Surgery 2003 76, 1246-1251DOI: (10.1016/S0003-4975(03)00721-5)

Fig 2 Collateralization as represented by Rentrop scores exhibited no significant change between the treatment and harvest time points for any of the three groups. Animals with incomplete occlusions had no development of collaterals and thus had scores of zero at both time points. (Del-1 = developmental endothelial locus-1; VEGF = vascular endothelial growth factor.) The Annals of Thoracic Surgery 2003 76, 1246-1251DOI: (10.1016/S0003-4975(03)00721-5)

Fig 3 Average regional blood flows (RBF) to area at risk in the control (n = 6), VEGF (n = 7), and Del-1 (n = 7) groups, respectively. RBF was significantly improved at stress (hearts paced at 180 bpm) compared with rest conditions in the VEGF group only. Figures are unitless indices, as interanimal variability in microsphere injections was standardized by dividing blood flow in the areas at risk by flow in uninvolved myocardium in the right ventricle. Hatched bars = rest; shaded bars = stress. (Del-1 = developmental endothelial locus-1; VEGF = vascular endothelial growth factor.) The Annals of Thoracic Surgery 2003 76, 1246-1251DOI: (10.1016/S0003-4975(03)00721-5)

Fig 4 Regional wall motion indices in control, Del-1-, and VEGF-treated animals. Animals treated with β-gal exhibited statistically significant deterioration in RWMI compared with either treatment group. Both Del-1 and VEGF groups showed no change except for a mild improvement from RWMI score of 2 to 3 in 1 Del-1-treated animal. (Del-1 = developmental endothelial locus-1; VEGF = vascular endothelial growth factor.) The Annals of Thoracic Surgery 2003 76, 1246-1251DOI: (10.1016/S0003-4975(03)00721-5)

Fig 5 Average global ejection fraction (GEF) as calculated using image analysis software of the left ventriculogram images. GEF significantly deteriorated in the β-gal-treated group between treatment and harvest time points (50.8 ± 6.8% versus 40.3 ± 3.7%, p = 0.02), whereas there was no significant reduction in the Del-1 (41.3 ± 11.2% versus 37.1 ± 7.5%, p = 0.26) or VEGF (45.4 ± 9.8% versus 46.6 ± 6.4%, p = 0.74) categories. Black bars = baseline; hatched bars = treatment; shaded bars = harvest. (Del-1 = developmental endothelial locus-1; VEGF = vascular endothelial growth factor.) The Annals of Thoracic Surgery 2003 76, 1246-1251DOI: (10.1016/S0003-4975(03)00721-5)

Fig 6 Regional preload recruitable stroke work (PRSW) as measured by sonomicrometry data. PRSW was significantly improved in the VEGF group (35.8 ± 8.6 versus 56.4 ± 17.8, p = 0.033). The improvement in the Del-1 category was not statistically significant (27.3 ± 9.8 versus 40.2 ± 19.4, p = 0.067). There was no change in this parameter of myocardial contractility in the β-gal treatment group (30.7 ± 14.8 versus 35.9 ± 12.1, p = 0.96). Hatched bars = treatment; shaded bars = harvest; black bar = noninfarct. (Del-1 = developmental endothelial locus-1; VEGF = vascular endothelial growth factor.) The Annals of Thoracic Surgery 2003 76, 1246-1251DOI: (10.1016/S0003-4975(03)00721-5)

Fig 7 Adenovirally mediated gene transfer of β-galactosidase as evidenced by blue staining at the 3-day and 1-week time points. Expression was lost at 2 weeks and there was no evidence of endogenous staining in the negative control sample. The Annals of Thoracic Surgery 2003 76, 1246-1251DOI: (10.1016/S0003-4975(03)00721-5)