Both SMN-FL and SMN-Δ7 proteins stabilized by the Z-FA-FMK treatment could form gem-like structures in Cajal bodies. Both SMN-FL and SMN-Δ7 proteins stabilized.

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Both SMN-FL and SMN-Δ7 proteins stabilized by the Z-FA-FMK treatment could form gem-like structures in Cajal bodies. Both SMN-FL and SMN-Δ7 proteins stabilized by the Z-FA-FMK treatment could form gem-like structures in Cajal bodies. (A, B) Representative immunostaining images illustrating the expression of Myc-SMN2a and Myc-SMN2d proteins in the DMSO- or Z-FA-FMK–treated groups at 8 h after doxycycline withdrawal. Nuclear Myc-SMN2a/d formed gems in Cajal bodies as indicated by double staining with Coilin (arrows). The third column in these panels indicated the enlarged view of the white-square–labeled area. Scale bars, 20 μm. (C, D) Quantification showing a significant increase in the number of both Myc-SMN2a (C) and Myc-SMN2d (D) gems after Z-FA-FMK treatment. Data were presented as mean ± SEM for Myc-SMN2a (n = 4) and Myc-SMN2d (n = 3). **P < 0.01, ***P < 0.001, as compared with the DMSO group by two-tailed t test. Yiran Wang et al. LSA 2019;2:e201800268 © 2019 Wang et al.