Endogenous SMN1 is not recruited to stress granules in HeLa cells after diverse stresses. Endogenous SMN1 is not recruited to stress granules in HeLa cells.

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Endogenous SMN1 is not recruited to stress granules in HeLa cells after diverse stresses. Endogenous SMN1 is not recruited to stress granules in HeLa cells after diverse stresses. (A) Extended panel from Fig 3D. HeLa cells, either untreated or incubated for 1 h at 44°C, were stained for EYFP (using a GFP antibody), endogenous SMN, and TIA-1 as marker for stress granules and analyzed by confocal microscopy. (B) HeLa cells, either untreated or incubated for 30 min with 0.5-mM arsenite, were stained for endogenous SMN and TIA-1 as marker for stress granules and analyzed by confocal microscopy. DAPI used to stain the nucleus. Scale bar indicates 20 μm. Jonathan Woodsmith et al. LSA 2018;1:e201800178 © 2018 Stelzl et al.