Analytical Evaluation of Primer Engineered Multiplex Polymerase Chain Reaction– Restriction Fragment Length Polymorphism for Detection of Factor V Leiden.

Slides:



Advertisements
Similar presentations
Measurement of Relative Copy Number of CDKN2A/ARF and CDKN2B in Bladder Cancer by Real-Time Quantitative PCR and Multiplex Ligation-Dependent Probe Amplification.
Advertisements

CyclinD1/CyclinD3 Ratio by Real-Time PCR Improves Specificity for the Diagnosis of Mantle Cell Lymphoma Carol D. Jones, Katherine H. Darnell, Roger A.
Design and Multiseries Validation of a Web-Based Gene Expression Assay for Predicting Breast Cancer Recurrence and Patient Survival Ryan K. Van Laar The.
Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.
Quantitative Detection and Differentiation of Human Herpesvirus 6 Subtypes in Bone Marrow Transplant Patients by Using a Single Real-Time Polymerase Chain.
A Prospective Clinical Study on the Use of Reverse Transcription-Polymerase Chain Reaction for the Early Diagnosis of Dengue Fever  Kamaljit Singh, Ajit.
A. Doménech-Sánchez, C. Juan, J.L. Pérez, C.I. Berrocal 
by S. Kangsadalampai, and P.G. Board
A. Doménech-Sánchez, C. Juan, J.L. Pérez, C.I. Berrocal 
Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis  Jeanne A. Jordan, Mary Beth.
Polymerase Chain Reaction
Microfluidic Platform for Single Nucleotide Polymorphism Genotyping of the Thiopurine S-Methyltransferase Gene to Evaluate Risk for Adverse Drug Events 
Comprehensive Analysis of CBFβ-MYH11 Fusion Transcripts in Acute Myeloid Leukemia by RT-PCR Analysis  ShriHari S. Kadkol, Annette Bruno, Carol Dodge,
Isothermal Multiple Displacement Amplification
Betaine, Dimethyl Sulfoxide, and 7-Deaza-dGTP, a Powerful Mixture for Amplification of GC-Rich DNA Sequences  Marco Musso, Renata Bocciardi, Sara Parodi,
Yanggu Shi, Sharon F. Terry, Patrick F. Terry, Lionel G
Rapid Simultaneous Screening of Factor V Leiden and G20210A Prothrombin Variant by Multiplex Polymerase Chain Reaction on Whole Blood by Encarnación Gómez,
Development and Evaluation of a Multiplex Real-Time Polymerase Chain Reaction Procedure to Clinically Type Prevalent Salmonella enterica Serovars  Nélida.
Laser-Assisted Microdissection of Membrane-Mounted Paraffin Sections for Polymerase Chain Reaction Analysis  Lise Mette Gjerdrum, Ilze Lielpetere, Lars.
Quantitative mRNA Expression Analysis from Formalin-Fixed, Paraffin-Embedded Tissues Using 5′ Nuclease Quantitative Reverse Transcription-Polymerase Chain.
Protocol for the Use of Polymerase Chain Reaction in the Detection of Intraocular Large B-Cell Lymphoma in Ocular Samples  Aires Lobo, Narciss Okhravi,
Long-Range (17.7 kb) Allele-Specific Polymerase Chain Reaction Method for Direct Haplotyping of R117H and IVS-8 Mutations of the Cystic Fibrosis Transmembrane.
Jeung-Yeal Ahn, Katie Seo, Olga Weinberg, Scott D. Boyd, Daniel A
Molecular Typing of West Nile Virus, Dengue, and St
PCR-RFLP detection and species identification of fungal pathogens in patients with febrile neutropenia  M. Dendis, R. Horváth, J. Michálek, F. Růžička,
Modulation of the expression of tissue plasminogen activator and its inhibitor by hypoxia in human peritoneal and adhesion fibroblasts  Ghassan M Saed,
Real-Time Polymerase Chain Reaction for Detecting Bacterial DNA Directly from Blood of Neonates Being Evaluated for Sepsis  Jeanne A. Jordan, Mary Beth.
Improved Real-Time Multiplex Polymerase Chain Reaction Detection of Methylenetetrahydrofolate Reductase (MTHFR) 677C>T and 1298A>C Polymorphisms Using.
Investigation of the human stem cell factor KIT ligand gene, KITLG, in women with 46,XX spontaneous premature ovarian failure  Emily S. Hui, B.A., Ekemini.
Clinical Laboratory Analysis of Immunoglobulin Heavy Chain Variable Region Genes for Chronic Lymphocytic Leukemia Prognosis  Philippe Szankasi, David.
Implications of Using the ND1 Gene as a Control Region for Real-Time PCR Analysis of Mitochondrial DNA Deletions in Human Skin  Andrew Harbottle, Kim.
Catherine E. Keegan, Anthony A. Killeen 
Heteroduplex Formation in SMN Gene Dosage Analysis
A 39-bp Deletion Polymorphism in PTEN in African American Individuals
Rapid and Inexpensive Detection of α1-Antitrypsin Deficiency-Related Alleles S and Z by a Real-Time Polymerase Chain Reaction Suitable for a Large-Scale.
Detection of FLT3 Internal Tandem Duplication and D835 Mutations by a Multiplex Polymerase Chain Reaction and Capillary Electrophoresis Assay  Kathleen.
Development of a Quantitative Real-Time Polymerase Chain Reaction Assay for the Detection of the JAK2 V617F Mutation  Elizabeth C. Wolstencroft, Katy.
The Molecular Pathology of Primary Immunodeficiencies
Validation and Clinical Application of a Locus-Specific Polymerase Chain Reaction- and Minisequencing-Based Assay for Congenital Adrenal Hyperplasia (21-Hydroxylase.
Allele-Specific, Non-Extendable Primer Blocker PCR (AS-NEPB-PCR) for DNA Mutation Detection in Cancer  Haiying Wang, John Jiang, Bianca Mostert, Anieta.
Detection of human telomerase reverse transcriptase mRNA in cells obtained by lavage of the pleura is not associated with worse outcome in patients with.
A Novel Semiquantitative Fluorescence-Based Multiplex Polymerase Chain Reaction Assay for Rapid Simultaneous Detection of Bacterial and Parasitic Pathogens.
A Multi-Exonic BRCA1 Deletion Identified in Multiple Families through Single Nucleotide Polymorphism Haplotype Pair Analysis and Gene Amplification with.
Novel Heat Pulse Extension-PCR–Based Method for Detection of Large CTG-Repeat Expansions in Myotonic Dystrophy Type 1  Arto K. Orpana, Tho H. Ho, Katariina.
Julie Di Cristofaro, Monique Silvy, Jacques Chiaroni, Pascal Bailly 
Characterization of Aberrant Melting Peaks in Unlabeled Probe Assays
Volume 119, Issue 4, Pages (October 2000)
Human Urine Contains Small, 150 to 250 Nucleotide-Sized, Soluble DNA Derived from the Circulation and May Be Useful in the Detection of Colorectal Cancer 
A Novel Long-Range PCR Sequencing Method for Genetic Analysis of the Entire PKD1 Gene  Ying-Cai Tan, Alber Michaeel, Jon Blumenfeld, Stephanie Donahue,
Rapid and Sensitive Real-Time Polymerase Chain Reaction Method for Detection and Quantification of 3243A>G Mitochondrial Point Mutation  Rinki Singh,
Amplification Refractory Mutation System, a Highly Sensitive and Simple Polymerase Chain Reaction Assay, for the Detection of JAK2 V617F Mutation in Chronic.
S.B. Schwartz, K.A. Thurman, S.L. Mitchell, B.J. Wolff, J.M. Winchell 
Novel Polymorphism in the FMR1 Gene Resulting in a “Pseudodeletion” of FMR1 in a Commonly Used Fragile X Assay  Thomas M. Daly, Arash Rafii, Rick A. Martin,
Template-Directed Dye-Terminator Incorporation with Fluorescence Polarization Detection for Analysis of Single Nucleotide Polymorphisms Implicated in.
Multiplex PCR Detection of GSTM1, GSTT1, and GSTP1 Gene Variants
Identification of Three Species of Borrelia burgdorferi Sensu Lato (B
X-Linked Anhidrotic (Hypohidrotic) Ectodermal Dysplasia Caused by a Novel Mutation in EDA1 Gene: 406T>G (Leu55Arg)  Francisco Martínez, José María Millán,
A Novel Multiplexing, Polymerase Chain Reaction-Based Assay for the Analysis of Chromosome 18q Status in Colorectal Cancer  Nadina Erill, Anna Colomer,
Rapid Polymerase Chain Reaction-Based Detection of Epidermal Growth Factor Receptor Gene Mutations in Lung Adenocarcinomas  Qiulu Pan, William Pao, Marc.
Genetic Analysis of Male Pattern Baldness and the 5α-Reductase Genes
Sensitive Detection of Polyalanine Expansions in PHOX2B by Polymerase Chain Reaction Using Bisulfite-Converted DNA  Hidekazu Horiuchi, Ayako Sasaki, Motoki.
Wook Lew  Journal of Investigative Dermatology 
Visual Format for Detection of Mycobacterium tuberculosis and M
A Prospective Clinical Study on the Use of Reverse Transcription-Polymerase Chain Reaction for the Early Diagnosis of Dengue Fever  Kamaljit Singh, Ajit.
Xiangfeng Cui, Helen Feiner, Honghua Li 
Detection of BRCA1 and BRCA2 Ashkenazi Jewish Founder Mutations in Formalin- Fixed Paraffin-Embedded Tissues Using Conventional PCR and Heteroduplex/Amplicon.
Detection and identification of bacterial DNA in semen
Angiotensin I-Converting Enzyme
Maureen J. O'Sullivan, Peter A. Humphrey, Louis P. Dehner, John D
Monique A. Johnson, Marvin J. Yoshitomi, C. Sue Richards 
Presentation transcript:

Analytical Evaluation of Primer Engineered Multiplex Polymerase Chain Reaction– Restriction Fragment Length Polymorphism for Detection of Factor V Leiden and Prothrombin G20210A  Suzanne Huber, Karolyn J. McMaster, Karl V. Voelkerding  The Journal of Molecular Diagnostics  Volume 2, Issue 3, Pages 153-157 (August 2000) DOI: 10.1016/S1525-1578(10)60631-9 Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Photograph of ethidium bromide-stained 3% agarose gel demonstrating primer-engineered PCR analysis for prothrombin G20210A and factor V Leiden. M denotes the molecular weight marker; W denotes water control.Lane 1: Undigested wild-type 506-bp (prothrombin) and 241-bp (factor V) amplicons.Lane 2: A prothrombin G20210/factor V wild type with digested products of 407, 241, and 99 bp. Lane 3: A prothrombin G20210 wild-type/factor V Leiden heterozygote with digestion products of 407, 241, 209, and 99 bp (32-bp product not shown). Lane 4: A prothrombin G20210 wild-type/factor V Leiden homozygote with digestion products of 407, 209, and 99 bp (32-bp product not shown). Lane 5: A prothrombin G20210A heterozygote/factor V Leiden heterozygote with digestion products of 407, 384, 241, 209, and 99 bp (32- and 23-bp products are not shown). The Journal of Molecular Diagnostics 2000 2, 153-157DOI: (10.1016/S1525-1578(10)60631-9) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Feedback: Privacy Policy Feedback
Do Not Sell My Personal Information
About project: SlidePlayer Terms of Service

© 2025 SlidePlayer.com. Inc. All rights reserved.