Epigallocatechin gallate, the main component of tea polyphenol, binds to CD4 and interferes with gp120 binding Kazushige Kawai, MD, Nelson H Tsuno, MD,

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Epigallocatechin gallate, the main component of tea polyphenol, binds to CD4 and interferes with gp120 binding Kazushige Kawai, MD, Nelson H Tsuno, MD, Joji Kitayama, MD, Yurai Okaji, MD, Kentaro Yazawa, MD, Masahiro Asakage, MD, Nobukazu Hori, MD, Toshiaki Watanabe, MD, Koki Takahashi, MD, Hirokazu Nagawa, MD Journal of Allergy and Clinical Immunology Volume 112, Issue 5, Pages 951-957 (November 2003) DOI: 10.1016/S0091-6749(03)02007-4

FIG 1 Flow-cytometric analysis of the expression of CD11a (A), CD3 (B), CD54 (C), and CD4 (D) on lymphocytes treated without (filled box) or with catechins at various concentrations (25 μmol/L EGCG [open box], 50 μmol/L EGCG [shaded box], or 50 μmol/L ECG [dotted box]). The data are expressed as the means ± SD of results from 3 independent experiments. ∗Statistical significance. Journal of Allergy and Clinical Immunology 2003 112, 951-957DOI: (10.1016/S0091-6749(03)02007-4)

FIG 2 The time course of CD4 downregulation after exposure to EGCG. Lymphocytes were exposed to EGCG at 50 μmol/L, and the expression intensity of CD4 was measured at the various time points by means of flow cytometry. The data are expressed as the means ± SD of results from 3 independent experiments. Journal of Allergy and Clinical Immunology 2003 112, 951-957DOI: (10.1016/S0091-6749(03)02007-4)

FIG 3 The CD4 expression of HL-60 (A) and U937 (B) incubated without (solid line) or with (dashed line) EGCG (50 μmol/L) evaluated by means of flow cytometry. Journal of Allergy and Clinical Immunology 2003 112, 951-957DOI: (10.1016/S0091-6749(03)02007-4)

FIG 4 Detection of CD4 endocytosis: CD4 expression on normal human T lymphocytes (A); acid treatment immediately after staining with FITC-conjugated anti-CD4 (B); 1-hour incubation of lymphocytes in medium before acid treatment (C); lymphocytes incubated with PMA (D) or EGCG (E) before acid treatment. The filled line represents the negative control. Journal of Allergy and Clinical Immunology 2003 112, 951-957DOI: (10.1016/S0091-6749(03)02007-4)

FIG 5 Western blot analysis of lymphocytes incubated without (left lane) or with ECG (middle lane) or EGCG (right lane). Membranes were stained with either anti-CD4 (upper) or anti-β-actin (lower). Journal of Allergy and Clinical Immunology 2003 112, 951-957DOI: (10.1016/S0091-6749(03)02007-4)

FIG 6 The competitive binding of EGCG and anti-CD4 antibody to the CD4 molecule analyzed by mean of ELISA. The vertical axis represents the percentage inhibition of the binding of anti-CD4 mAb to the CD4 molecule and was obtained on the basis of the ratio to the control wells. Data are expressed as the mean ± SD of results from 3 wells. Journal of Allergy and Clinical Immunology 2003 112, 951-957DOI: (10.1016/S0091-6749(03)02007-4)

FIG 7 EGCG interference with GP120 binding to CD4. The vertical axis represents the mean fluorescence intensity of FITC-conjugated gp120 bound to lymphocytes obtained by means of flow cytometry. The data are expressed as mean ± SD of results from 3 independent experiments. ∗Statistical significance. Journal of Allergy and Clinical Immunology 2003 112, 951-957DOI: (10.1016/S0091-6749(03)02007-4)