Changes in mutation rate or protein abundance are not observed in HATs when comparing rho+ to rho0 cells. Changes in mutation rate or protein abundance.

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Changes in mutation rate or protein abundance are not observed in HATs when comparing rho+ to rho0 cells. Changes in mutation rate or protein abundance are not observed in HATs when comparing rho+ to rho0 cells. (A) The sequence of the listed genes obtained based on deep sequencing of two independent samples of input DNA was queried against the reference HG19 genome. The number of base changes (single nucleotide polymorphisms [SNPs]) identified relative to the sequence contained in the reference HG19 genome is shown for each sample. The number of SNPs identified in the rho0 samples that were not identified in the rho+ control is also shown. For those, the genomic location and the exact base change is depicted (last column). (B) Western blots probing levels of the two main HATs that acetylate H3K9 and/or H3K27 was performed in whole cell lysates of rho+ or rho0 cells. RNA pol II was used as loading control. Oswaldo A Lozoya et al. LSA 2019;2:e201800228 © 2019 Lozoya et al.