Deletion of Srf with a skeletal muscle-specific Cre transgene.

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Presentation transcript:

Deletion of Srf with a skeletal muscle-specific Cre transgene. Deletion of Srf with a skeletal muscle-specific Cre transgene. (A) Myo-Cre transgenic mice were bred with ROSA26R indicator mice to determine the temporal and tissue specificity of Cre expression. Whole-mount photographs of β-galactosidase-stained embryos of the indicated embryonic ages are shown. The lacZ reporter gene is activated specifically in the skeletal muscle lineage. (B) WT and Srfflex1/flex1/Myo-Cre (KO) mice immediately after birth are shown. The mutant is cyanotic and displays curvature of the spine. (C) The structure of the Srfflex1 allele before (Upper) and after (Lower) Cre-mediated recombination is shown. Triangles represent loxP sites. Exons 1 and 2 are shown in black boxes with the 5′ UTR as a white box. Primers used by PCR are designated L and R, and sizes of PCR fragments are indicated. (D) PCR of genomic DNA from skeletal muscle of mice of the indicated genotypes. Primers L and R yield a product of 1,340 bp with the Srfflex1 allele and 380 bp with the Srf/x1 allele in the presence of the Myo-Cre transgene. Shijie Li et al. PNAS 2005;102:4:1082-1087 ©2005 by National Academy of Sciences