Figure 2 Induced deletion of CXCR2 on oligodendrocyte lineage cells after tamoxifen injection in Cxcr2-cKO mice Induced deletion of CXCR2 on oligodendrocyte.

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Figure 2 Induced deletion of CXCR2 on oligodendrocyte lineage cells after tamoxifen injection in Cxcr2-cKO mice Induced deletion of CXCR2 on oligodendrocyte lineage cells after tamoxifen injection in Cxcr2-cKO mice (A) Striatum from ROSA26 mT/mG+:PLPCreERT+ mice with or without tamoxifen injection (4 weeks after injection) reveals the expression of tomato red and GFP by fluorescence microscopy. These data represent at least 3 independent experiments. Scale bar: 25 μM, n = 3 mice each experiment. (B) Brain cells from B6, ROSA26 mT/mG+:PLPCreERT+ mice with or without tamoxifen injection were isolated by Percoll gradient, stained with CD45 antibody, and analyzed by flow cytometry. These data represent at least 3 independent experiments. (C) Brain cells from control and Cxcr2-cKO mice 4 weeks after tamoxifen injection were isolated by Percoll gradient. The PLPeGFP-positive cells were sorted from 6 pooled mice per group. Total RNA was extracted from sorted cells and qPCR was performed for CXCR2 and PLP genes. These data represent 3 technical replicates. LiPing Liu et al. Neurol Neuroimmunol Neuroinflamm 2015;2:e174 © 2015 American Academy of Neurology