Mast cells regulate IFN-γ expression in the skin and circulating IgE levels in allergen- induced skin inflammation Harri Alenius, PhD, Dhafer Laouini,

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Mast cells regulate IFN-γ expression in the skin and circulating IgE levels in allergen- induced skin inflammation Harri Alenius, PhD, Dhafer Laouini, PhD, Amy Woodward, MD, Emiko Mizoguchi, MD, PhD, Atul K. Bhan, MD, Emanuela Castigli, PhD, Hans C. Oettgen, MD, PhD, Raif S. Geha, MD Journal of Allergy and Clinical Immunology Volume 109, Issue 1, Pages 106-113 (January 2002) DOI: 10.1067/mai.2002.120553 Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 1 A, Histologic features of OVA-sensitized and saline-sensitized skin sites in W/Wv mice and controls. Magnification: large panels, ×200; small panels, ×1000. Arrowheads point to eosinophils. There is thickening of epidermal layer in the OVA-sensitized skin sites from W/Wv and WT mice in comparison with saline-sensitized skin sites. Infiltration of eosinophils and mononuclear cells is increased in OVA-sensitized skin sites in comparison with saline sensitization. B, Number of infiltrating mononuclear cells in OVA- and saline-exposed skin sites from W/Wv and WT mice. C, Number of infiltrating eosinophils. Cellular infiltrate in the dermis was assessed by examining 15 TO 20 HPFs and calculating the mean number of infiltrating eosinophils or mononuclear cells. The columns and error bars represent means ± SEMs. *P < .05. ***P < .001. Journal of Allergy and Clinical Immunology 2002 109, 106-113DOI: (10.1067/mai.2002.120553) Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 2 IL-4 and IFN-γ mRNA levels in biopsy specimens from skin sites of W/Wv and WT mice sensitized with OVA or saline, expressed relative to mRNA levels of β-2-microglobulin as assessed by RT-PCR analysis. These are pooled results of experiments with OVA-sensitized mice (W/Wv group, n = 7; WT group, n = 11) and saline-sensitized controls (n = 9 in both W/Wv and WT groups), each run being performed in duplicate. The columns and error bars represent means ± SEMs. *P < .05. **P < .01. ***P < .001. Journal of Allergy and Clinical Immunology 2002 109, 106-113DOI: (10.1067/mai.2002.120553) Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 3 Baseline expression of IL-4, IL-10, and IL-12 in mRNA levels in biopsy specimens from unsensitized skin of W/Wv and WT mice (each group, n = 5), expressed relative to mRNA levels of β-2-microglobulin as assessed by RT-PCR analysis. These are pooled results of experiments with 5 W/Wv and 5 WT mice. The columns and error bars represent means ± SEMs. *P < .05. **P < .01. Journal of Allergy and Clinical Immunology 2002 109, 106-113DOI: (10.1067/mai.2002.120553) Copyright © 2002 Mosby, Inc. Terms and Conditions

Fig. 4 Serum IgE and serum antibody responses in W/Wv mice and controls sensitized with OVA or saline solution. A, Total serum IgE. Baseline IgE (BASE) and IgE after saline (SAL) or ovalbumin (OVA) sensitization. B, OVA-specific IgE levels, expressed in nanograms per milliliter relative to an OVA-specific mAb standard. C, OVA-specific IgG2a antibody levels expressed in units relative to a serum pool of intraperitoneally immunized mice run in each assay. Each dot represent 1 animal (n = 7 to 11 animals per group). *P < .05. **P < .01. ***P < .001. Journal of Allergy and Clinical Immunology 2002 109, 106-113DOI: (10.1067/mai.2002.120553) Copyright © 2002 Mosby, Inc. Terms and Conditions