NsiR4 is involved in regulating NH4+ assimilation. NsiR4 is involved in regulating NH4+ assimilation. Schema illustrating the N-regulated feed-forward mechanism for the direct regulation of the glutamine synthetase inactivating factor IF7, thereby also affecting GS activity. AMT, ammonium transporter; NRT, nitrate transporter; GS, glutamine synthetase; GOGAT, glutamine oxoglutarate amidotransferase (glutamate synthase); NR, nitrate reductase; NiR, nitrite reductase; CM, cytoplasmic membrane. Stephan Klähn et al. PNAS 2015;112:45:E6243-E6252 ©2015 by National Academy of Sciences