Tapetal mitochondria in wild-type (WT), drp3b, and elm1 mutants.

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Tapetal mitochondria in wild-type (WT), drp3b, and elm1 mutants. Tapetal mitochondria in wild-type (WT), drp3b, and elm1 mutants. A to C, Confocal images of mitochondria in a BN-stage tapetal cell expressing mito-GFP. Scale bars = 10 μm. D, Quantification of total mitochondrial volume per tapetal cell. E, Mitochondrial size (the volume of individual mitochondria). F, Total mitochondria number. G, The average skeleton cross-section area of tapetal mitochondria. H, Mitochondrial distance to centroid of the whole mitochondrial population in cells. I, Mean of mitochondrial pairwise distance. At least 20 tapetal cells of each genotype at each stage were quantified from three biological experiments. The results are shown as mean ± se. The statistically significant difference (P < 0.05) between stages of each genotype is indicated by a different letter according to Student’s t test. Within a stage, significant difference (P < 0.05) of mutants versus wild type and between two mutants are labeled with “*” and “#”, respectively. Pei-Ying Chen et al. Plant Physiol. 2019;180:813-826 ©2019 by American Society of Plant Biologists