Mineral content analysis by SEM-EDX.

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Mineral content analysis by SEM-EDX. Mineral content analysis by SEM-EDX. A, Representative SEM cross section of root with labeled arrows indicating the cell types whose elemental composition was analyzed by EDX. Scale bar = 0.1 mm. B, Percentage of total counts of Na+ and K+, measured in 4 to 5 cells of indicated cell types from 2 plants of each genotype (Exod, exodermis; Endo, endodermis; ParX, xylem parenchyma). Plants were treated with 50 mm NaCl for 2 d. Shown are the mean and SD values. Letters indicate significantly different values relative to that in the wild type (WT) for each cellular type determined by one-way ANOVA (a = P < 0.01; b = P < 0.05). C, Na/K ratios of net content values given in (B). The experiment was repeated twice with similar results. Houda El Mahi et al. Plant Physiol. 2019;180:1046-1065 ©2019 by American Society of Plant Biologists