Volume 56, Issue 4, Pages (October 1999)

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Volume 56, Issue 4, Pages 1310-1312 (October 1999) Oxidative stress mediates apoptosis induced by oxidized low-density lipoprotein and oxidized lipoprotein(a) Kathrin Heermeier, Reinhard Schneider, Alexandra Heinloth, Christoph Wanner, Stephanie Dimmeler, Jan Galle Kidney International Volume 56, Issue 4, Pages 1310-1312 (October 1999) DOI: 10.1046/j.1523-1755.1999.00685.x Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 1 Apoptosis in isolated intact segments of rabbit aorta, intraluminally perfused with oxidized low density lipoprotein (oxLDL, 300 μg/ml; A) and oxidized lipoprotein(a) [oxLp(a), 30 μg/ml; B] for 6, 12, or 24 hours, the enhancement of apoptosis by the SOD inhibitor diethyl-dithio-carbamate (DDC, 10mm), and the inhibition of apoptosis by the O2--metabolizing enzymes SOD (0.1 μm) and catalase (100 U/ml). Apoptosis was determined by TUNEL staining, and the rate of apoptosis is expressed as a percentage of TUNEL-positive cells. Data are means ± se of four experiments. Symbols in A are: (□) control; () oxLDL; () oxidized LDL + DDC; () oxidized LDL + DDC + SOD/cat. Symbols in B are: (□) control; () oxLp(a); () oxidized Lp(a) + DDC; () oxidized Lp(a) + DDC + SOD/cat. *P < 0.05 oxLDL vs. oxLDL + DDC, respectively. oxLp(a) vs. oxLp(a) + DDC; #P < 0.05 oxLDL + DDC vs. oxLDL + DDC + SOD/catalase, respectively. oxLp(a) + DDC vs. oxLp(a) + DDC + SOD/catalase. Kidney International 1999 56, 1310-1312DOI: (10.1046/j.1523-1755.1999.00685.x) Copyright © 1999 International Society of Nephrology Terms and Conditions