Anaphylaxis to apple and orange seed

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Presentation transcript:

Anaphylaxis to apple and orange seed Paul J. Turner, FRACP, PhD, Paul E.A. Gray, FRACP, Melanie Wong, FRACP, PhD, Nirupama Varese, MSc, Jennifer M. Rolland, PhD, Robyn O’Hehir, FRACP, PhD, Dianne E. Campbell, FRACP, PhD Journal of Allergy and Clinical Immunology Volume 128, Issue 6, Pages 1363-1365 (December 2011) DOI: 10.1016/j.jaci.2011.07.005 Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Analysis of apple and other protein extracts. Peanut (P), sesame seed (S), apple seed (AS), apple pulp (AP), and apple-containing baby food (B) extracts were resolved under reducing conditions with 4% to 12% SDS-PAGE and stained with Coomassie brilliant blue. Mr, Molecular mass markers. Journal of Allergy and Clinical Immunology 2011 128, 1363-1365DOI: (10.1016/j.jaci.2011.07.005) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 2 IgE immunoblotting of peanut (P), sesame seed (S), apple seed (AS), and apple pulp (AP) extracts. Extracts were resolved with 4% to 12% SDS-PAGE under reducing conditions and then transferred to nitrocellulose and probed with the patient’s serum at a 1:5 dilution. Mr, Molecular mass markers. Journal of Allergy and Clinical Immunology 2011 128, 1363-1365DOI: (10.1016/j.jaci.2011.07.005) Copyright © 2011 American Academy of Allergy, Asthma & Immunology Terms and Conditions