IWGS workflow. iWGS workflow. A typical iWGS analysis consists of four steps: (1) data simulation (optional); (2) preprocessing (optional); (3) de novo.

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iWGS workflow. iWGS workflow. A typical iWGS analysis consists of four steps: (1) data simulation (optional); (2) preprocessing (optional); (3) de novo assembly; and (4) assembly evaluation. iWGS supports both Illumina short reads and PacBio long reads, and a wide selection of assemblers to enable de novo assembly using either or both types of data. Users can start the analysis simulating data drawn from a reference genome assembly or, alternatively, use real sequencing data as input and skip the simulation step. iWGS, in silico Whole Genome Sequencer and Analyzer; MP, mate-pair; PacBio, Pacific Biosciences; PE, paired-end. Xiaofan Zhou et al. G3 2016;6:3655-3662 ©2016 by Genetics Society of America