OCT and ERG analysis of wild-type mice 2 and 4 wk after injection with AAVs. OCT and ERG analysis of wild-type mice 2 and 4 wk after injection with AAVs.

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OCT and ERG analysis of wild-type mice 2 and 4 wk after injection with AAVs. OCT and ERG analysis of wild-type mice 2 and 4 wk after injection with AAVs. (A) Fundus photography and direct GFP fluorescence detection 2 wk postinjection of the AAVs in WT C57Bl/6J animals. The site of injection is marked by an asterisk (*, red). (B) Representative OCT scans indicating normal morphology and lamination 1 mo postinjection with AAV-GFP and AAV-Cre. (C) Quantification of total retinal thickness indicating no change in GFP- versus Cre-transduced WT animals (n = 5). (D) Scotopic ERG measurements of the a-, b-, and c-wave amplitudes and implicit time 1 mo postinjection (n = 12). Error bars are means ± SEM. Unpaired t tests were used in all comparisons. No statistical difference was determined between the cohorts. Mariana Aparecida Brunini Rosales et al. LSA 2019;2:e201800212 © 2019 Rosales et al.