Figure 5 Electron microscopy of transfected HeLa cells and Dnm1Ftfl neurons (A) Electron microscopy (EM) of HeLa cells transfected with wild type (WT)

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Figure 2. Co-expression of mutant proteins with wt cx26

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Nat. Rev. Neurol. doi: /nrneurol

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Figure 5 Electron microscopy of transfected HeLa cells and Dnm1Ftfl neurons (A) Electron microscopy (EM) of HeLa cells transfected with wild type (WT) and mutant DNM1 constructs. Electron microscopy of transfected HeLa cells and Dnm1Ftfl neurons (A) Electron microscopy (EM) of HeLa cells transfected with wild type (WT) and mutant DNM1 constructs. There are larger and abnormal vesicles in the A177P mutant. Cells transfected with the G359A show no obvious vesicle defects. Scale bars, 1 μm. (B) EM of WT mouse brain sections. (C) EM of Dnm1Ftfl mouse neurons reveals increased vesicle size. (D) A box plot depicting the number of vesicles in both WT and Dnm1Ftfl neurons. Outliers are excluded from the plot. Significance was determined by the Mann-Whitney-Wilcoxon test (**p < 0.005, ***p < 0.0005). (E) A box plot depicting the size of vesicles in both WT and Dnm1Ftfl neurons. There were significantly larger vesicles in both hippocampal and cortical neurons. Outliers are excluded from the plot. Significance was determined by the Mann-Whitney-Wilcoxon test. Ryan S. Dhindsa et al. Neurol Genet 2015;1:e4 © 2015 American Academy of Neurology