AAF/II does not mask colonization-associated phenotypes produced by Hra1. AAF/II does not mask colonization-associated phenotypes produced by Hra1. (A)

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AAF/II does not mask colonization-associated phenotypes produced by Hra1. AAF/II does not mask colonization-associated phenotypes produced by Hra1. (A) Biofilm formation of EAEC strain 042 and its hra1 and aafA mutants (SB1 and 3.4.14, respectively) and of aggR mutant JS28 in high-glucose DMEM at 8 h in the presence and absence of AAF/II fimbrial assembly inhibitor NTZ. Biofilm formation was measured on a logarithmic scale as the absorbance at 570 nm of crystal violet eluted from a stained and fixed biofilm. With the exception of hra1 mutant SB1, all of the tested mutants produced significantly less-copious biofilm than 042 (P < 0.01). NTZ significantly reduced biofilm formation by 042 and SB1 (P < 0.02) but not 3.4.14, JS28, or negative-control E. coli K-12 strain MG1655. (B) Autoaggregation of 042 (solid diamonds), SB1 (Δhra1; crossed lines), 3.4.14 (ΔaafA; solid squares), and MG1655 (K-12 control; open squares) in high-glucose DMEM. Laura V. Blanton et al. mSphere 2018; doi:10.1128/mSphere.00078-18