Investice do rozvoje vzdělávání Micromanipulation (Metody pro mikrosopickou manipulaci s nanokonstrukty) Zbyněk Heger 28.2.2014 Investice do rozvoje vzdělávání

Micromanipulation Very small structures, concurrently enlarged via microscope. Commercially used for assisted fertilization. Many possibilities in nano-, micro-scale research. 2

Assisted fertilization In vitro fertilization (IVF). Louise Joy Brown – 1. IVF born by IVF (25. 6. 1978). Hormonal induction of superovulation (E2 levels). Oocytes picking up Fertilization (intracytoplasmic injection of sperm – ICSI – low sperm motility, small number of oocytes, immunological infertility) Embryo transfer 3

Devices Inverted microscope with programmable micromanipulators, Microinjector, Microelectrode, Piezoelectric microdrill, Software. 4

Patch clamp Neher and Sakmann - the Nobel Prize in Physiology or Medicine in 1991 Microelectrophysiology ´´Patch microelectrodes´´ Current of single ion channels – connection in fundamental cell processes Spheroplasts Patch clamp record 5

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Micromanipulation Maimon et al., Nature Neuroscience. 2013. 7

Micromanipulation Semyanov et al., Nature. 2008. 8

Micromanipulation Sridharan et al., Journal of Neural engineering. 2013. 9

Micromanipulation Pavenstadt et al., American Journal of Physiology. 2013. 10

Investice do rozvoje vzdělávání Acknowledgements Investice do rozvoje vzdělávání 11

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