Actin’ on HIV: How Dendritic Cells Spread Infection Daniel A. Donahue, Olivier Schwartz  Cell Host & Microbe  Volume 19, Issue 3, Pages 267-269 (March 2016) DOI: 10.1016/j.chom.2016.02.016 Copyright © 2016 Elsevier Inc. Terms and Conditions

Figure 1 Interaction of a Dendritic Cell with a CD4 T Cell An immature human DC (blue) is shown interacting with a CD4 T cell (yellow). Image is from O. Schwartz and Institut Pasteur. Cell Host & Microbe 2016 19, 267-269DOI: (10.1016/j.chom.2016.02.016) Copyright © 2016 Elsevier Inc. Terms and Conditions

Figure 2 Regulation of Actin Dynamics in Dendritic Cells Influences trans Infection (Top) In DCs (blue), a stable cortical actin barrier (black border) beneath the cell membrane limits endocytosis. HIV particles (green) are captured by the DC and maintained on dendrites. These virions are efficiently transmitted to CD4 T cells (red). In the absence of Tetraspanin 7 (middle) or Dynamin 2 (bottom), cortical actin is destabilized. This results in higher rates of endocytosis and internalization of HIV via macropinocytosis. Dendrites are unaffected when Dynamin 2 is silenced. The DC forms blebs instead of dendrites upon Tetraspanin 7 silencing. In both cases, HIV particles are internalized and trans infection of CD4 T cells is diminished. Cell Host & Microbe 2016 19, 267-269DOI: (10.1016/j.chom.2016.02.016) Copyright © 2016 Elsevier Inc. Terms and Conditions