Genetic FIP200 deletion impairs autophagy induction and causes T cell apoptosis. Genetic FIP200 deletion impairs autophagy induction and causes T cell.

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Genetic FIP200 deletion impairs autophagy induction and causes T cell apoptosis. Genetic FIP200 deletion impairs autophagy induction and causes T cell apoptosis. (A) FIP200 protein expression in T cell subsets and non–T cells from Fip200flox/floxCd4+/+ (WT), Cd4c/+, and Cd4c/c (KO) mice (n = 3). (B and C) Spontaneous apoptosis of naïve T cells in WT and Fip200−/− mice. (B) Numbers on the dot plots represent the percentage of Annexin V+ naïve T cells in lymph nodes. (C) The percentages of CD4+ and CD8+ Annexin V+ naïve T cells are shown (n = 5, means ± SEM). *P < 0.05 (Mann-Whitney U tests). (D and E) Apoptosis of cultured or activated T cells in WT and Fip200−/− mice. Naïve lymph node T cells were cultured for 24 and 48 hours without (D) or with (E) anti-CD3 and anti-CD28 antibodies. Numbers on the dot plots represent the percentage of Annexin V+ T cells (n = 4, means ± SEM). *P < 0.05 (Mann-Whitney U tests). (F) The percentage of T cell subsets in lymph nodes (LNs; n = 6), spleen (SP; n = 5 to 7), bone marrow (BM; n = 3), and peripheral blood (PB; n = 3) in WT and Fip200−/− mice. *P < 0.05 (Mann-Whitney U tests). (G) Absolute numbers of T cell subsets in spleen from WT and Fip200−/− mice (n = 13, means ± SEM). *P < 0.05 (Mann-Whitney U tests). (H and I) WT CD45.1+ T cells and Fip200−/− CD45.2+ T cells were transferred into Rag1−/− mice with a 1:1 ratio. Live (H) and apoptotic (I) T cells were analyzed on day 14 in the lymph nodes and spleen in Rag1−/− mice. Numbers on the dot plots represent the percentage of CD45.1+ and CD45.2+ T cells in total T cells (H) and the percentage of Annexin V+ T cells in WT and Fip200−/− (KO) T cells (I) (n = 7). (J) Autophagy flux in T cells isolated from lymph nodes of WT and KO mice with or without TCR engagement for 24 hours. These T cells were treated with Baf for the last 4 hours to inhibit the autophagosome degradation (n = 3). (K) Expression of autophagy receptor p62 and NDP52 in fresh WT and Fip200−/− naïve T cell subsets (n = 3). Houjun Xia et al. Sci. Immunol. 2017;2:eaan4631 Copyright © 2017 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works