Morphologic and functional evaluation of adult conditional PGC-1 knockout mice. Morphologic and functional evaluation of adult conditional PGC-1 knockout.

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Morphologic and functional evaluation of adult conditional PGC-1 knockout mice. Morphologic and functional evaluation of adult conditional PGC-1 knockout mice. (A) Representative OCT images from AAV-GFP and AAV-Cre–injected PGC-1A;B–floxed mice 1 and 4 mo postinduction. (B) Quantification of total retinal thickness from OCT imaging (n = 5–7). (C) Scotopic ERG recordings at a luminance of 0.246 (P) cd.s/m2 from AAV-GFP (blue) and AAV-Cre (red) animals. (D) Quantification of a- and b-wave amplitudes for increasing stimulus intensity at 4 mo (n = 5). (E) Implicit times of a- and b-wave at light stimulus of 0.246 (P) cd.s/m2 at 4 mo (n = 5). (F) RPE-generated c-wave amplitude and implicit time in AAV GFP group mice (blue) compared with AAV-GFP injected mice (red) measured with a light stimulus of 24.1 cd.s/m2 at 4 mo (n = 6). Error bars are means ± SEM. Data were analyzed by unpaired t test. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001 compared with their respective AAV-GFP controls. Mariana Aparecida Brunini Rosales et al. LSA 2019;2:e201800212 © 2019 Rosales et al.