Silencing Ifngr1 in the CMT167 line confers decreased response to IFNγ in vitro and in vivo. Silencing Ifngr1 in the CMT167 line confers decreased response.

Slides:

Advertisements

Similar presentations

Clancy-Thompson et al., Supplemental Figure 1 A B C Supplemental Figure 1: Mice were given intravenous B16 injection on day 0. A) After bearing tumors.

Advertisements

Glioma-derived soluble factor(s) enhance IL-10 signaling.

From: CXCL10 Is Required to Maintain T-Cell Populations and to Control Parasite Replication during Chronic Ocular Toxoplasmosis Invest. Ophthalmol. Vis.

Aldehyde Dehydrogenase 1A1 Possesses Stem-Like Properties and Predicts Lung Cancer Patient Outcome Xiao Li, MD, Liyan Wan, MD, Jian Geng, MD, Chin-Lee.

Volume 15, Issue 6, Pages (June 2009)

Volume 30, Issue 2, Pages (August 2016)

Volume 43, Issue 4, Pages (October 2015)

Expression of α5β1-integrin enhances EboV GP-mediated infection of GD25 cells. Expression of α5β1-integrin enhances EboV GP-mediated infection of GD25.

Altered cell migration factors and increased intestinal tumorigenesis in APC1638N/+ mice. Altered cell migration factors and increased intestinal tumorigenesis.

by R. Dixon Dorand, Joseph Nthale, Jay T. Myers, Deborah S

Fig. 1. Potent and selective down-regulation of KRAS mRNA and protein by AZD4785 in vitro and in vivo. Potent and selective down-regulation of KRAS mRNA.

NPM1 drives poly-GR into the nucleolus of primary neurons.

[68Ga]Pentixafor PET imaging of MM xenografts Real‐time PCR analysis of cxcr4 transcript expression levels in HeLa (negative control) and in MM cell lines.

Sunitinib plus rMVA–CEA–TRICOM vaccine decreased tumor burden and increased intratumoral infiltration of T lymphocytes in the MC38-CEA colon carcinoma.

Aldehyde Dehydrogenase 1A1 Possesses Stem-Like Properties and Predicts Lung Cancer Patient Outcome Xiao Li, MD, Liyan Wan, MD, Jian Geng, MD, Chin-Lee.

Volume 30, Issue 2, Pages (August 2016)

TGF‐β1 and Sema3A are downregulated in vivo by increasing VEGF doses Muscles were harvested 7 days after implantation of V Low, V Med, and V High clones.

Fig. 4. aPD-1 mAb transfer to macrophages is mediated by FcγRs.

H31m1-PDL1 cells form progressively growing tumors in WT mice.

Figure S2 Supplementary data a b CCA1 CCA2 CCA4 CCA3 CCA5 EGFR HER2

Relative mRNA expression level (/J3T-1)

Volume 152, Issue 1, Pages (January 2019)

Volume 93, Issue 1, (January 2018)

Microarray analysis of phospho-Twist1–responsive genes.

TSA dose-dependent increase in transcription.

Fig. 5. pKL cells abrogate the autoimmune response in vitro.

Fig. 5 Local gel scaffold for T cell memory response.

Volume 17, Issue 2, Pages (August 2015)

Molecular Therapy - Nucleic Acids

Volume 43, Issue 2, Pages (August 2015)

BDNF expression in the cerebellum and brain stem region.

Volume 48, Issue 4, Pages e4 (April 2018)

Wei-Yun Lai, Bo-Tsang Huang, Jen-Wei Wang, Pei-Ying Lin, Pan-Chyr Yang

LFA-1 is present in cytosolic clusters similar to those containing RhoB and tubulin in migrating T lymphocytes, and reducing RhoB abundance impairs the.

Fig. 2. IL-2/rapamycin–expanded T cells express homing receptors to traffic to lymphoma sites and are resistant to SN-38 toxicity. IL-2/rapamycin–expanded.

Impaired TrkB signaling in the cerebellum of Pex14ΔC/ΔC BL/ICR mice.

Volume 46, Issue 1, Pages (January 2017)

Therapeutic activity of gal‐encapsulated cytotoxic drugs on tumor xenografts Therapeutic activity of gal‐encapsulated cytotoxic drugs on tumor xenografts.

Volume 32, Issue 6, Pages (June 2010)

Socs1 KD tumors show alterations in multiple populations.

Gold Nanoparticles for BCR-ABL1 Gene Silencing: Improving Tyrosine Kinase Inhibitor Efficacy in Chronic Myeloid Leukemia Raquel Vinhas, Alexandra R.

LLC cells exhibit a blunted response to IFNγ in vitro and in vivo compared with CMT167. LLC cells exhibit a blunted response to IFNγ in vitro and in vivo.

TSA causes premature chromatid sister separation.

SEMA3C regulates prostate cancer cell growth

Identification of Z-FA-FMK as a potent compound that increases SMN protein expression in HEK293 and patient fibroblast cells. Identification of Z-FA-FMK.

IFN-γ and TNF-α cause an upregulation of Fas expression in human ESCs

Caspase 8 is expressed in human ESCs but is not regulated by IFN-γ, TNF-α or hCG. Caspase 8 is expressed in human ESCs but is not regulated by IFN-γ, TNF-α.

Socs1 KD tumors exhibit a more T-cell–inflamed phenotype and increased T-cell activation. Socs1 KD tumors exhibit a more T-cell–inflamed phenotype and.

Socs1 KD tumors exhibit a more T cell–inflamed phenotype and increased CD8+ T cell activation. Socs1 KD tumors exhibit a more T cell–inflamed phenotype.

NK cell function of spleen MNCs (A) and NK cell frequencies in the ileum, duodenum, and spleen (B) of protein-deficient and -sufficient HIFM/HRV-infected.

Silencing Socs1 in the LLC line confers increased response to IFNγ in vitro. Silencing Socs1 in the LLC line confers increased response to IFNγ in vitro.

NOX1-deficient mice do not respond to GKT771 and exhibit reduced B16-F10 tumor growth associated with decreased production of angiogenic factors and minor.

Metformin and rapamycin decreased tumor weights in obese prediabetic mice. Metformin and rapamycin decreased tumor weights in obese prediabetic mice. A:

PTX in combination with PLX397 induces antitumor T-cell response.

MK-8628 restricts cytokine production in T lymphocytes.

Silencing Socs1 in the LLC line confers increased response to IFNγ in vitro and in vivo. Silencing Socs1 in the LLC line confers increased response to.

CD151 effects on mammary cell morphology and adhesion.

Analysis of tumor-infiltrating CD8+ T cells and effect of T and NK cell depletion. Analysis of tumor-infiltrating CD8+ T cells and effect of T and NK cell.

Ctip2 directly regulates Notch1 expression in differentiated cells.

Overexpression of IL-27 upregulates expression of multiple IR by T cells in vivo. Overexpression of IL-27 upregulates expression of multiple IR by T cells.

Increased CCL5 expression, infiltration of Treg cells, and apoptosis of CD8+ T cells in human colorectal tissues. Increased CCL5 expression, infiltration.

EMT gene expression patterns of M-Wnt and E-Wnt cells in vitro and in vivo. EMT gene expression patterns of M-Wnt and E-Wnt cells in vitro and in vivo.

Volume 25, Issue 6, Pages (June 2017)

SY-1425 induces maturation in RARA-high AML

NT157 treatment inhibits LNCaP xenograft growth and delays castration-resistant progression. NT157 treatment inhibits LNCaP xenograft growth and delays.

Fig. 6 Antitumor effect on tumor growth and pulmonary metastasis of CSSD-9 in vivo. Antitumor effect on tumor growth and pulmonary metastasis of CSSD-9.

Expression of IFN-λs, IFN-λR1, and IL-10R2 in Xenopus tissues after stimulation in vivo with poly(I:C). Expression of IFN-λs, IFN-λR1, and IL-10R2 in Xenopus.

Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells. Coculture with U937 cells enhances DNMT1 expression in gastric cancer cells.

CD36 expression in tissue adjacent and distal to the tumor.

EC-derived SP cells are targeted by CD30.CAR T cells.

Presentation transcript:

Silencing Ifngr1 in the CMT167 line confers decreased response to IFNγ in vitro and in vivo. Silencing Ifngr1 in the CMT167 line confers decreased response to IFNγ in vitro and in vivo. (A, B) In vitro mRNA expression of (A) Ifngr1 and (B) Ifngr2 in FPKM as assessed by RNA-Seq. (C) Immunoblots of IFNGR1 and IFNGR2 expression relative to β-ACTIN levels in LLC and CMT167 cells in vitro. (D–I) Full data sets for mRNA expression are shown for (D) Ifngr1, (E) Cxcl9, (F) Cxcl10, (G) Cd274, (H) Ciita, and (I) Socs1 after ±10 ng/ml IFNγ for 24 h. Statistics compare cells lines treated with IFNγ relative to the CMT-NT cell line. (J) PD-L1 protein expression (MFI) of CMT-NT versus CMT-sh68sc3 cells in vitro by flow cytometry after ±10 ng/ml IFNγ treatment for 72 h. Statistics compare all groups. (K, L) Representative images of H&E staining of tumors from the following experimental conditions: (K) CMT-NT IgG2a and CMT-NT PD-1 and (L) CMT-sh68sc3 IgG2a and CMT-sh68sc3 PD-1 (scale bars: 100 μm and magnified to 10 μm). Tumors are dense and stain dark purple relative to adjacent normal lung tissue. Red arrows mark lymphocyte nests. Error bars represent the mean of the data ± SEM after a t test (S2A, B) or a two-way ANOVA (D–J) (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001). Source data are available for this figure. Bonnie L Bullock et al. LSA 2019;2:e201900328 © 2019 Bullock et al.