Antibody reactivity against HA1 proteins after vaccination.

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Presentation transcript:

Antibody reactivity against HA1 proteins after vaccination. Antibody reactivity against HA1 proteins after vaccination. Anti-IgG (A, B, and C) and anti-IgA (D and E) antibody responses after boosting with H5 vaccine are depicted as floating bar graphs or as Tukey box plots. Bar graphs are sorted by subtype and decreasing signal intensity (SI) at d28. Each bar represents the difference between the average signal intensities of two time points for a strain (blue dots, d0 SI; red dots, d28 SI; error bars, standard deviations [SD], n = 10). Dark bars indicate a positive difference between the two time points (d28 > d0), while light bars indicate a negative difference (d0 > d28). A heat map of delta values (d28 − d0) is shown above each graph. The orange area represents the signal intensity distribution (average ± 1 SD, n = 11) of a reference group (GCRC) with no active influenza virus infection or history of H5 vaccination. The Tukey box plots in panels B and E show H5 alone and pooled group 1 or group 2 subtype signal intensities. Panel C shows average signal intensities at the two time points among the various H5 clades. Means are indicated by “+.” The two-tailed Mann-Whitney test for unpaired data was used to calculate statistical significance between the two time points, where P < 0.05 defines statistical significance. HA1, hemagglutinin head domain; d0, day 0; d28, day 28; GCRC, General Clinical Research Center (UC Irvine). Rie Nakajima et al. mSphere 2018; doi:10.1128/mSphere.00592-18