Volume 6, Issue 1, Pages 76-87 (January 2013) Strigolactones and the Regulation of Pea Symbioses in Response to Nitrate and Phosphate Deficiency  Eloise Foo, Kaori Yoneyama, Cassandra J. Hugill, Laura J. Quittenden, James B. Reid  Molecular Plant  Volume 6, Issue 1, Pages 76-87 (January 2013) DOI: 10.1093/mp/sss115 Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 1 Effects of Nitrate and Phosphate Withdrawal on Wild-Type and rms4 Pea Plants.(A) Strigolactone levels in root exudate, as monitored by UPLC/MS, and (B) shoot and root fresh weight. Plants were grown in hydroponics for 10 d with control nutrient or nutrient lacking nitrogen (–N) or lacking phosphate (–P). Values are mean ± s.e., n = 3; n.d. indicates no signal was detected. Molecular Plant 2013 6, 76-87DOI: (10.1093/mp/sss115) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 2 Strigolactone Levels in Wild-Type and Pea Mutants (ccd8, rms2, rms4, ccd7). Data come from two different experiments measuring (A) root exudate and (B) root tissue of 3-week-old plants grown under low phosphate (0.05 mM NaH2PO4), as monitored by UPLC/MS; n.d. indicates no signal was detected. Values are mean ± s.e., n = 4. Molecular Plant 2013 6, 76-87DOI: (10.1093/mp/sss115) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 3 Mycorrhizal Colonization of Roots of Wild-Type and Pea Mutants (ccd8, rms2, and rms4). Plants were grown for 6 weeks in pot culture with Glomus intraradices under low phosphate (0.05 mM NaH2PO4). Parvus is the wild-type background for ccd8, while Torsdag is the wild-type background for rms2 and rms4 mutants. Experiments using different genetic backgrounds were carried out at different times. Values are mean ± s.e., n = 3–6. Molecular Plant 2013 6, 76-87DOI: (10.1093/mp/sss115) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 4 Effect of Phosphate Fertilization on Mycorrhizal Root Colonization of Wild-Type and Strigolactone Mutants (ccd8 and rms4). (A) Percentage of root colonized and (B) percentage of root that had arbuscules present. Plants were grown for 6 weeks in pot culture with Glomus intraradices and treated three times a week with nutrient solution containing 0.05 or 5 mM NaH2PO4. n.d. indicates no arbuscules were detected. Values are mean ± s.e., n = 6–9. Molecular Plant 2013 6, 76-87DOI: (10.1093/mp/sss115) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 5 Nodulation of Wild-Type and Pea Mutants (ccd7, ccd8, rms2, and rms4) under Nitrate-Free Conditions.(A, B) Nodule number per gram root dry weight of 3-week-old plants, (C) nodule number per gram root dry weight of 2-, 3-, and 4-week-old plants, and (D) photo of nodules on secondary root of wild-type and rms4 plants (tertiary roots have been removed); scale bar = 1 cm. (A), (B) and (C) represent three experiments. (D) represents the same experiment as for (B). For (A–C), values are mean ± s.e., n = 5–6. Molecular Plant 2013 6, 76-87DOI: (10.1093/mp/sss115) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 6 Nodulation of Roots of Reciprocal Grafts between Wild-Type and rms4Plants were grown under nitrate-free conditions. Nodule number per gram root dry weight of plants 4 weeks after grafting. Values are mean ± s.e., n = 5–8. Molecular Plant 2013 6, 76-87DOI: (10.1093/mp/sss115) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 7 Effect of Nitrate Fertilization on Nodulation of Wild-Type and ccd8 Strigolactone-Deficient Mutants. Plants were grown for 3 weeks and treated three times a week with nutrient solution containing 0 or 10 mM KNO3.(A) Nodule number per gram root dry weight, (B) average nodule size, and (C) photo of nodules on secondary roots of wild-type plants (tertiary roots have been removed), scale bar = 1 cm. For (A, B), values are mean ± s.e., n = 5–6. Molecular Plant 2013 6, 76-87DOI: (10.1093/mp/sss115) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions

Figure 8 Effect of Phosphate Fertilization on Nodulation of Wild-Type and ccd8 Strigolactone-Deficient Mutants. Plants were grown for 3 weeks and treated three times a week with nutrient solution containing 0.05 or 5 mM NaH2PO4.(A) Nodule number per gram root dry weight, (B) average nodule dry weight. Values are mean ± s.e., n = 6–9. Molecular Plant 2013 6, 76-87DOI: (10.1093/mp/sss115) Copyright © 2013 The Authors. All rights reserved. Terms and Conditions