IL-6 neutralization results in induction of CD161+ FOXP3+ CD127– Tregs in EC–CD4+ T cell cocultures. IL-6 neutralization results in induction of CD161+

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IL-6 neutralization results in induction of CD161+ FOXP3+ CD127– Tregs in EC–CD4+ T cell cocultures. IL-6 neutralization results in induction of CD161+ FOXP3+ CD127– Tregs in EC–CD4+ T cell cocultures. CD4+ CD45RA− memory T cells were cocultured for 7 d with allogeneic HLA-DR+ ECs in the presence of an IL-6 neutralizing Ab (30 ug/ml) or isotype control. Multiparameter flow cytometric analysis was performed for CD161, CD25, FOXP3, CD127, and Helios in freshly isolated cells or after coculture for 7 d. A, Flow cytometric analysis for CD25 and FOXP3 expression on CD161+-gated CD4+ CD45RA+ memory T cells. *p = 0.002. B, Flow cytometric analysis for CD127 and Helios expression on CD161+ CD25+ FOXP3+-gated cells. Birgit Fogal et al. J Immunol 2011;187:6268-6280 Copyright © 2011 by The American Association of Immunologists, Inc.