Titration of antisera against the amount of extract loaded on Western blots. Titration of antisera against the amount of extract loaded on Western blots.

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Titration of antisera against the amount of extract loaded on Western blots. Titration of antisera against the amount of extract loaded on Western blots. Slots of a 15% SDS polyacrylamide gel were loaded with increasing numbers of A280 units of whole cell lysate from BY4741 grown in YEP-galactose medium. After electrophoresis and blotting, the membrane was probed with a cocktail of antisera against uS4, uL4, uL5, or uL18. The intensity of the bands was determined using ImageJ, and the relative band intensities are plotted against the number of A280 units loaded in each slot. Brian Gregory et al. LSA 2019;2:e201800150 © 2019 Gregory et al.