Video-imaging microfluorometry identifies α- and β-like cell types in Madin-Darby canine kidney monolayers Stephanie Ebner, Marcos Marin-Grez Kidney.

Slides:

Advertisements

Similar presentations

Volume 63, Issue 4, Pages (April 2003)

Advertisements

Pathophysiology of renal fluid retention

Bcl-2 overexpression prevents apoptosis-induced Madin-Darby canine kidney simple epithelial cyst formation1 Hsi-Hui Lin, Tzi-Peng Yang, Si-Tse Jiang,

Volume 55, Issue 4, Pages (April 1999)

Effect of arginine vasopressin and ANP on intracellular pH and cytosolic free [Ca2+] regulation in MDCK cells Maria Oliveira-Souza, Margarida Mello-Aires,

Calcium phosphate–induced renal epithelial injury and stone formation: Involvement of reactive oxygen species Kinue Aihara, Karen J. Byer, Saeed R. Khan

Volume 79, Issue 8, Pages (April 2011)

Rudolfs K. Zalups, Sarfaraz Ahmad Kidney International

The C-terminal tail of aquaporin-2 determines apical trafficking

Ruisheng Liu, Oscar A. Carretero, Yilin Ren, Jeffrey L. Garvin

Volume 60, Issue 3, Pages (September 2001)

Calcium phosphate–induced renal epithelial injury and stone formation: Involvement of reactive oxygen species Kinue Aihara, Karen J. Byer, Saeed R. Khan

Michael Fritzenwanger, Ines Heid, Adolf Dörge Kidney International

Volume 56, Issue 2, Pages (August 1999)

Volume 79, Issue 8, Pages (April 2011)

Volume 68, Issue 4, Pages (October 2005)

Making sense of the sensor: Mysteries of the macula densa

Progression of renal failure and hypertensive nephrosclerosis

Bcl-2 overexpression prevents apoptosis-induced Madin-Darby canine kidney simple epithelial cyst formation1 Hsi-Hui Lin, Tzi-Peng Yang, Si-Tse Jiang,

Pathophysiology of renal fluid retention

Volume 60, Issue 4, Pages (October 2001)

Sulfite-mediated oxidative stress in kidney cells

Volume 73, Issue 2, Pages (January 2008)

Volume 64, Issue 1, Pages 2-10 (July 2003)

Volume 66, Issue 5, Pages (November 2004)

Volume 55, Issue 6, Pages (June 1999)

Volume 68, Issue 4, Pages (October 2005)

Volume 61, Issue 1, Pages S3-S8 (January 2002)

Bcl-2 overexpression prevents apoptosis-induced Madin-Darby canine kidney simple epithelial cyst formation1 Hsi-Hui Lin, Tzi-Peng Yang, Si-Tse Jiang,

Volume 130, Issue 6, (September 2007)

The epidemiology of chronic kidney disease

Spontaneous self-alkalinization in spontaneous tumor lysis syndrome

Volume 61, Issue 1, Pages S106-S109 (January 2002)

Public health in Africa: What is new—The context, the gains, the losses, the renewed public health, and the way forward D.A.N.C.O. Kaseje, Pamela Juma,

Reverse transcriptase-polymerase chain reaction study of anion exchanger-2 in canine tissues and different Madin-Darby canine kidney cell types Graham.

Volume 69, Issue 12, Pages (June 2006)

Volume 69, Issue 3, Pages (February 2006)

Kian Hong Ng, Beng Gek Lim, Kim Ping Wong Kidney International

Microbiology and outcomes of peritonitis in North America

Calcium sensing in podocytes

AGEs in foods: Do they play a role in uremia?

Volume 63, Issue 4, Pages (April 2003)

Nephrology Crossword: Glomerulonephritis

Fluorescent imaging of acute mercuric chloride exposure on cultured human kidney tubular epithelial cells Zhichao Zhang, Xiangfeng Guo, Xuhong Qian,

Volume 55, Issue 4, Pages (April 1999)

Volume 60, Issue 2, Pages (August 2001)

A man with a hole in his penis

Lu-Cheng Cao, Thomas Honeyman, Julie Jonassen, Cheryl Scheid

Volume 64, Issue 2, Pages (August 2003)

Organ transplantation goes to the movies

Volume 53, Issue 5, Pages (May 1998)

Modulation of renal epithelial barrier function by mitogen-activated protein kinases (MAPKs): Mechanism of cyclosporine A–induced increase in transepithelial.

Volume 55, Pages S3-S16 (June 1999)

Tyrosine kinase inhibitors and immunosuppressants perturb the myo-inositol but not the betaine cotransporter in isotonic and hypertonic MDCK cells Mohamed.

Ionic transport in macula densa cells

A new regulator of the vacuolar H+-ATPase in the kidney

Volume 79, Issue 12, Pages (June 2011)

Influence of extracellular pH on intracellular pH in arterioles and skeletal muscle of spontaneously hypertensive and Wistar-Kyoto rats Pauline Wimberger,

Effect of lipid-lowering strategies on tubular cell biology

Charles A. Herzog Kidney International

Katherine K. Rogers, Tzuu-Shuh Jou, Wei Guo, Joshua H. Lipschutz

Volume 72, Issue 4, Pages (August 2007)

Volume 70, Issue 3, Pages (August 2006)

Volume 70, Issue 5, Pages (September 2006)

Volume 82, Issue 9, Pages (November 2012)

Volume 55, Issue 1, Pages (January 1999)

Development and differentiation of endothelium

Volume 134, Issue 6, (September 2008)

Yasuo Kohjimoto, Lori Kennington, Cheryl R. Scheid, Thomas W. Honeyman

Volume 55, Issue 1, Pages (January 1999)

Presentation transcript:

Video-imaging microfluorometry identifies α- and β-like cell types in Madin-Darby canine kidney monolayers Stephanie Ebner, Marcos Marin-Grez Kidney International Volume 54, Pages S139-S142 (September 1998) DOI: 10.1046/j.1523-1755.1998.06727.x Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 1 Images were obtained, as described in the text, from wild-type Madin-Darby canine kidney (MDCK) cells loaded with c-SNAFL-2 and excited at 520 ± 2nm. (A) A confluent monolayer 24hours after seeding on a cell culture dish (×40). (B) A confluent monolayer 24hours after seeding on a cell culture insert (×20). (C) Subcellular fluid of a dome obtained after a 20minutes loading period (×40). (D) A confluent monolayer loaded with c-SNAFL-2-DA after preincubation with 4-(chloromercuri)phenylsulfonic acid (PCMBS). Kidney International 1998 54, S139-S142DOI: (10.1046/j.1523-1755.1998.06727.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 2 Histogram of gray-value intensity of 968 wild-type MDCK cells (5 cell-culture dishes) loaded with c-SNAFL-2 and excited at 520 ± 2nm. Kidney International 1998 54, S139-S142DOI: (10.1046/j.1523-1755.1998.06727.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 3 Intracellular pH measurements of Madin-Darby canine kidney (MDCK) cells 24 after seeding. The [Cl-] (inmm) of the apical and basolateral perfusate is shown in the box over the curves. (A) The results from the highly fluorescent cells (**P < 0.01), and (B) those from the medium fluorescent cells (***P < 0.001). Each point is the mean of 20 cells (except for the first control measurement lower panel, N = 16). Kidney International 1998 54, S139-S142DOI: (10.1046/j.1523-1755.1998.06727.x) Copyright © 1998 International Society of Nephrology Terms and Conditions