Earlier Studies Background: Laminin is one of the basement proteins.

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Presentation transcript:

Earlier Studies Background: Laminin is one of the basement proteins. EX18404 (東京工業大学推薦課題) 飯田　慎仁（大阪大学蛋白質研究所）全原子分子動力学法を用いた細胞外マトリックス蛋白質の相互作用解析 Background: Laminin is one of the basement proteins. Basement membranes are ubiquitous 1 Plasma membrane Extracellular Basement membrane Intracellular Binds Cell attachment LG1 LG2 LG3 g-tail Integrin recognition region (LamininE8) Integrin Laminin Question: How does the laminin E8 recognize an integrin? Earlier Studies X-ray structure: Laminin E8 Missing g-tail: FNTPSIEKP LG1 LG2 LG3 The Glu1607 is positioned at a region in an integrin. Hypothesis Laminin E8 exposes Glu1607 to the binding site of the integrin ahead of time? Integrin-binding activity could benefit from such structures. By disulfide bond screening for the tail peptide and an integrin. M. Takizawa et al. Sci. Adv., 2017, 3, e1701497. Materials and Methods water Na+ Cl- LG1 LG2 g-tail I use all-atom molecular dynamics (MD) simulation combined with a generalized ensemble (GE) method in order to enhance structural sampling beyond that of conventional MD. The GE method is called virtual-system coupled Multicanonical Molecular Dynamics (V-McMD). I have already prepared a simulated system of laminin E8 (Right figure), which is being used in our simulations. Future Plan By performing V-McMD simulations, a variety of structures of laminin E8 will be obtained, and that hypothesis will be verified. Moreover, I will show that my computational results would predict unknown phenomena for interaction of laminin E8 with an integrin. The prediction will be confirmed by my collaborators who will perform experiments. I would like to thank Takizawa Mamoru, Taniguchi Yukimasa, and Sekiguchi Kiyotoshi