Transcriptome analysis of miR-210Δ mutant flies revealed potential miR-210 targets. Transcriptome analysis of miR-210Δ mutant flies revealed potential.

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Transcriptome analysis of miR-210Δ mutant flies revealed potential miR-210 targets. Transcriptome analysis of miR-210Δ mutant flies revealed potential miR-210 targets. (A) 812 differentially expressed RNAs were identified in 1 h old miR-210Δ heads compared with control by RNA sequencing (*adjusted P value < 0.05, n = 3). (B) GO enrichment of differentially regulated mRNAs demonstrated that many genes with GO terms related to phototransduction and vision were down-regulated. (C) Four potential miR-210 targets predicted by PicTar and TargetScan were also up-regulated on RNA level in miR-210Δ mutant flies. (D) The receptor potential of miR-210Δ mutants was partially rescued by Fasn1 RNAi as measured by ERG in 4-d-old flies (miR-210Δ; GMR-Gal4/+ versus miR-210Δ; and GMR-Gal4>UAS-Fasn1 RNAi: *P < 0.05, n = 7–9). (E) Photoreceptors in miR-210Δ; GMR-Gal4> Fasn1 RNAi showed a similar grade of retinal degeneration compared with miR-210Δ controls. (F) ERG showed that the receptor potential of miR-210Δ mutants was significantly increased by overexpression of bmm lipase (miR-210Δ; GMR-Gal4/+ versus miR-210Δ; GMR-Gal4>UAS-bmm: ***P < 0.001; miR-210Δ; UAS-bmm/+ versus miR-210Δ; and GMR-Gal4>UAS-bmm: *P < 0.05, n = 4–5). Carina M Weigelt et al. LSA 2019;2:e201800149 © 2019 Weigelt et al.