Tissue-specific transcriptome responses to fasting.

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Tissue-specific transcriptome responses to fasting. Tissue-specific transcriptome responses to fasting. (A) Examples of heat maps from RNAseq libraries showing induction (red) or repression (green) of distinct gene groups in specific tissues of female, wild-type (w1118) flies after 6 h of starvation. (B) Gene Ontology enrichment analysis of genes induced by starvation in distinct tissues. (C) Average fold induction of the top 50 genes most strongly induced in oenocytes/epidermal cells. Average/SD is shown from RNAseq results of three independent samples. See fully labeled chart in Fig. S1B. (D) Quantitative RT-PCR to validate the induction of pepck, CG5966, and bmm in oenocyte/epidermal samples of female flies after 6 h of starvation. Expression values are normalized to actin5C. Averages and SEM are shown. P values are from Student t test (n ≥ 3). See also Figs. S1 and S2, Dataset S1, and SI Experimental Procedures. Debamita Chatterjee et al. PNAS 2014;111:50:17959-17964 ©2014 by National Academy of Sciences