Distribution of phosphosites and PDGFRα activity in primary cultures.

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Distribution of phosphosites and PDGFRα activity in primary cultures. Distribution of phosphosites and PDGFRα activity in primary cultures. (A) The distribution of the number of phosphorylation sites per protein along with the average size of proteins (amino acid numbers) for the 5,767 phosphosites from the PDGFRα MS dataset. (B–F) Quantitative Westerns blot analysis of PDGFRα autophosphorylation tyrosine residues for four primary cultures of PDGFRα-driven GBMs. Cells were treated with 10 μg/ml doxycycline for 48 h or 25 ng/ml of recombinant PDGF-AA for 15 min in serum-deficient media. Source data are available for this figure. Shuang Zhou et al. LSA 2018;1:e201800029 © 2018 zhou et al.