Volume 55, Issue 6, Pages 2407-2414 (June 1999) Cyclosporine A up-regulates angiotensin II receptors and calcium responses in human vascular smooth muscle cells  Pavel V. Avdonin, Florence Cottet-Maire, Galina V. Afanasjeva, Svetlana A. Loktionova, Philippe Lhote, Urs T. Ruegg  Kidney International  Volume 55, Issue 6, Pages 2407-2414 (June 1999) DOI: 10.1046/j.1523-1755.1999.00481.x Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 1 Effects of cyclosporine A (CsA) on free cytoplasmic calcium concentration ([Ca2+]c) (A) and on Ang II-induced [Ca2+]c increments over basal level (B) in human smooth muscle cells (SMCs). Cells were pretreated in the presence of 1 and 10 μm CsA or 0.1% ethanol (control, CT) during 24hours. The results shown in (A) are representative of five to eight experiments. Lines are: (solid line) CsA 10 μm; (long dash) control; (short dash) CsA 1 μm; bars represent the means ± sem. *P < 0.05; **P < 0.01. Kidney International 1999 55, 2407-2414DOI: (10.1046/j.1523-1755.1999.00481.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 2 Influence of CsA on angiotensin II (Ang II)-induced increment in 45Ca2+ efflux (Δ 45Ca2+). Cells were pretreated for 24hours with the indicated concentrations of CsA. The data are normalized to Ang II-induced increment in 45Ca2+ efflux over basal in cells not treated with CsA. Each point represents the mean ± sem of three to four experiments performed in quadruplicate. *P < 0.05; **P < 0.02; #P < 0.01. Significance (P values) was determined with respect to control (CT). Kidney International 1999 55, 2407-2414DOI: (10.1046/j.1523-1755.1999.00481.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 3 (A) Specific binding of [125I]Ang II (0.12nM) to SMCs treated with vehicle or 10 μM CsA for 24hours (mean ± SEM of eight measurements performed in quadruplicate). (B) Scatchard plot for [125I]Ang II binding to control (CT; □) and CsA-treated (•) SMCs. Each point of the plot was obtained from three to six experiments performed in quadruplicate (*P<0.02). Kidney International 1999 55, 2407-2414DOI: (10.1046/j.1523-1755.1999.00481.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 4 Influence of actinomycin D (1 μM) on the CsA effects on Ang II-induced 45Ca2+ efflux (A) and [125I]Ang II (0.12nM) binding (B). Data are mean ± sem from three experiments performed in quadruplicate. *P < 0.01; **P < 0.05; NS, not significant. Kidney International 1999 55, 2407-2414DOI: (10.1046/j.1523-1755.1999.00481.x) Copyright © 1999 International Society of Nephrology Terms and Conditions

Figure 5 Comparison of the effects of CsA and CsH on Ang II-induced 45Ca2+ efflux. Cells were pretreated with 10 μm CsA or CsH, respectively, for 24hours. The data are mean ± sem from three experiments performed in quadruplicate (*P < 0.02; **P < 0.01). Kidney International 1999 55, 2407-2414DOI: (10.1046/j.1523-1755.1999.00481.x) Copyright © 1999 International Society of Nephrology Terms and Conditions