Encapsulation of CpG-C ISS in two pH-sensitive liposome preparations.

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Encapsulation of CpG-C ISS in two pH-sensitive liposome preparations. Encapsulation of CpG-C ISS in two pH-sensitive liposome preparations. The liposomes were prepared from mixtures of CHEMS, DOTAP, and PE as described in Materials and methods and purified through gel filtration on a 9-ml Superose 6 column equilibrated with the 0.125 M NaCl, 0.01 M Hepes, pH 8.4 buffer. (A–C) Representation of absorbance monitored at 310 nm (thick line) and 260 nm (thin line) over time is shown. ODN are detected at 260 nm only while the liposomes can be detected at both 260 and 310 nm. (A) Representation of one liposome preparation mixed with free ODN. (B) Representation of one liposome preparation stable until pH < 5.0. (C) Representation of another liposome preparation stable until pH < 6.0. Cristiana Guiducci et al. J Exp Med 2006;203:1999-2008 © 2006 Rockefeller University Press