Sevoflurane preconditioning-induced neuroprotection is associated with Akt activation via carboxy-terminal modulator protein inhibition Y. Chen, H. Nie,

Slides:

Advertisements

Similar presentations

Tetrahydrocurcumin provides neuroprotection in rats after traumatic brain injury: autophagy and the PI3K/AKT pathways as a potential mechanism Yongyue.

Advertisements

Fig. 2. Neuroprotective effects of ex-4 through the G protein-coupled GLP-1 receptor. (A) Infarct volume was assessed by TTC staining after tMCAO in rats.

Postischemic administration of liposome-encapsulated luteolin prevents against ischemia-reperfusion injury in a rat middle cerebral artery occlusion model

Sevoflurane pre-conditioning increases phosphorylation of Erk1/2 and HO-1 expression via inhibition of mPTP in primary rat cortical neurons exposed to.

The novel exercise-induced hormone irisin protects against neuronal injury via activation of the Akt and ERK1/2 signaling pathways and contributes to.

Cerebrovasc Dis 2016;42: DOI: /

Contribution of nitric oxide synthase (NOS) in blood–brain barrier disruption during acute focal cerebral ischemia in normal rat Mohammad T. Mohammadi,

Vascular PPARδ Protects Against Stroke-Induced Brain Injury

J. Chen, W. Chen, M. Zhu, Y. Zhu, H. Yin, Z. Tan

Dexmedetomidine, an α-2a adrenergic agonist, promotes ischemic tolerance in a murine model of spinal cord ischemia-reperfusion Marshall T. Bell, MD,

Hyperphosphorylation of microtubule-associated tau protein plays dual role in neurodegeneration and neuroprotection Yao Zhang, Qing Tian, Qi Zhang, Xinwen.

The mechanisms of cardio-protective effects of desflurane and sevoflurane at the time of reperfusion: anaesthetic post-conditioning potentially translatable.

Blockade of spinal nerves inhibits expression of neural growth factor in the myocardium at an early stage of acute myocardial infarction in rats W. Yue,

H. Wang, Y. Dong, J. Zhang, Z. Xu, G. Wang, C. A. Swain, Y. Zhang, Z

Inhibition of the Let-7 Family MicroRNAs Induces Cardioprotection Against Ischemia- Reperfusion Injury in Diabetic Rats Juchen Li, MD, Yixing Ren, MD,

Volume 56, Issue 3, Pages (March 2012)

Myocardial ischaemia reperfusion injury: the challenge of translating ischaemic and anaesthetic protection from animal models to humans Z. Xia, H. Li,

Isoflurane-induced myocardial preconditioning is dependent on phosphatidylinositol-3- kinase/Akt signalling J. Raphael, J. Rivo, Y. Gozal British Journal.

X. Sun, J. Ellis, P.J. Corso, P.C. Hill, F. Chen, J. Lindsay

Mu and delta, but not kappa, opioid agonists induce spastic paraparesis after a short period of spinal cord ischaemia in rats† M Kakinohana, S Nakamura,

J.R. Liu, C. Baek, X.H. Han, P. Shoureshi, S.G. Soriano

Translocation of protein kinase C isoforms is involved in propofol-induced endothelial nitric oxide synthase activation L. Wang, B. Wu, Y. Sun, T. Xu,

Type of anaesthesia and patient quality of recovery: a randomized trial comparing propofol–remifentanil total i.v. anaesthesia with desflurane anaesthesia

Combined treatment with celecoxib and sevoflurane after global cerebral ischaemia has no additive neuroprotective effects in rats J.H. Seo, H.P. Park,

Isoflurane and sevoflurane increase interleukin-6 levels through the nuclear factor- kappa B pathway in neuroglioma cells L. Zhang, J. Zhang, L. Yang,

L. Tong, M. Cai, Y. Huang, H. Zhang, B. Su, Z. Li, H. Dong

Dexmedetomidine-mediated neuroprotection against sevoflurane-induced neurotoxicity extends to several brain regions in neonatal rats J.F. Perez-Zoghbi,

T. Annecke, D. Chappell, C. Chen, M. Jacob, U. Welsch, C. P

Sevoflurane promotes the expansion of glioma stem cells through activation of hypoxia- inducible factors in vitro Q.Y. Shi, S.J. Zhang, L. Liu, Q.S. Chen,

The essential role of endothelial nitric oxide synthase activation in insulin-mediated neuroprotection against ischemic stroke in diabetes Shiang-Suo.

G. Xu, H. Lu, Y. Dong, D. Shapoval, S. G. Soriano, X. Liu, Y. Zhang, Z

B. Haelewyn, A. Yvon, J. L. Hanouz, E. T. MacKenzie, P. Ducouret, J. L

General anaesthetics do not impair developmental expression of the KCC2 potassium- chloride cotransporter in neonatal rats during the brain growth spurt

JNK mediates hepatic ischemia reperfusion injury

Triaging the right patient to the right place in the shortest time

Thrombus in left ventricle discovered by transoesophageal echocardiography (TOE) in a patient with acute abdomen: how TOE can be crucial for decision-making.

Sevoflurane-induced cardioprotection depends on PKC-α activation via production of reactive oxygen species R.A. Bouwman, R.J.P. Musters, B.J. van Beek-Harmsen,

D. Ebel, P. Lipfert, J. FraÕßdorf, B. Preckel, J. MuÕllenheim, V

Pretreatment with intrathecal or intravenous morphine attenuates hepatic ischaemia– reperfusion injury in normal and cirrhotic rat liver Y. Wang, G.T.C.

Dexmedetomidine post-treatment induces neuroprotection via activation of extracellular signal-regulated kinase in rats with subarachnoid haemorrhage

A. Vincelot, N. Nathan, D. Collet, Y. Mehaddi, P. Grandchamp, A. Julia

Timing of xenon-induced delayed postconditioning to protect against spinal cord ischaemia–reperfusion injury in rats Y.W. Yang, W.P. Cheng, J.K. Lu,

Evolution of cerebral ischaemia induced by thromboembolism in rats detected by early sequential MR imaging S. Ishikawa, K. Yokoyama, T. Kuroiwa, K. Makita

Sevoflurane post-conditioning increases nuclear factor erythroid 2-related factor and haemoxygenase-1 expression via protein kinase C pathway in a rat.

R Huhn, A Heinen, N.C. Weber, W Schlack, B Preckel, M.W. Hollmann

Inhibiting NADPH oxidase protects against long-term memory impairment induced by neonatal sevoflurane exposure in mice Z. Sun, M. Satomoto, Y.U. Adachi,

S. Pilge, D. Jordan, M. Kreuzer, E.F. Kochs, G. Schneider

Obal D. , Weber N. C. , Zacharowski K. , Toma O. , Dettwiler S

Resistin promotes CCL4 expression through toll-like receptor-4 and activation of the p38-MAPK and NF-κB signaling pathways: implications for intervertebral.

Anaesthesia for awake craniotomy—evolution of a technique that facilitates awake neurological testing A. Sarang, J. Dinsmore British Journal of Anaesthesia

Remifentanil and cyclooxygenase inhibitors interactions in the minimum alveolar concentration of sevoflurane in the rat J Benito, D Aguado, M.B. Abreu,

Systemic oxygen extraction during exercise at high altitude

Hyperglycaemia blocks sevoflurane-induced postconditioning in the rat heart in vivo: cardioprotection can be restored by blocking the mitochondrial permeability.

Effects of different remifentanil target concentrations on MAC BAR of sevoflurane in gynaecological patients with CO2 pneumoperitoneum stimulus Z.-Y.

Experimental behaviour testing: pain

P. Fogarty Mack British Journal of Anaesthesia

Proposal for a surrogate surgical invasiveness score to obtain a ‘post hoc’ quantification of surgical stress and tissue trauma in the context of postoperative.

Pulmonary overexpression of inhibitor κBα decreases the severity of ventilator-induced lung injury in a rat model M. Hayes, G.F. Curley, C. Masterson,

Methylnaltrexone to prevent intrathecal morphine-induced pruritus after Caesarean delivery: a multicentre, randomized clinical trial M. Paech, B. Sng,

Neurological biomarkers in the perioperative period

Suspected opioid-induced hyperalgesia in an infant

Volume 22, Issue 10, Pages (October 2014)

Cross-tolerance between spinal neostigmine and morphine in the rat

Effects of propofol on the expression of matric metalloproteinases in rat cardiac fibroblasts after hypoxia and reoxygenation J.H. Jun, J.E. Cho, Y.H.

Regional anaesthesia to improve pain outcomes in paediatric surgical patients: a qualitative systematic review of randomized controlled trials S. Suresh,

Cardioprotection by sevoflurane against reperfusion injury after cardioplegic arrest in the rat is independent of three types of cardioplegia D. Ebel,

Helium-induced late preconditioning in the rat heart in vivo†

Cannabinoid 1 receptor mediation of spinal cord ischemic tolerance induced by limb remote ischemia preconditioning in rats Binxiao Su, MD, Hailong Dong,

D. Obal, B. Preckel, H. Scharbatke, J. Müllenheim, F. Höterkes, V

Resveratrol neuroprotective effects during focal cerebral ischemia injury via nitric oxide mechanism in rats Shen-Kou Tsai, MD, PhD, Li-Man Hung, PhD,

Presentation transcript:

Sevoflurane preconditioning-induced neuroprotection is associated with Akt activation via carboxy-terminal modulator protein inhibition Y. Chen, H. Nie, L. Tian, L. Tong, J. Deng, Y. Zhang, H. Dong, L. Xiong British Journal of Anaesthesia Volume 114, Issue 2, Pages 327-335 (February 2015) DOI: 10.1093/bja/aeu271 Copyright © 2015 The Author(s) Terms and Conditions

Fig 1 (a) Graphical representation of the protocol for experiment I shows that neurological deficits and infarct volumes were evaluated at 1 and 7 days after reperfusion to investigate the neuroprotective effect of sevoflurane preconditioning against focal cerebral ischaemia. Expression of pSer473-Akt, pSer9 GSK-3β, and CTMP were analysed at different time points after reperfusion. (b) Graphical representation of the protocol for experiment II shows that PI3K inhibitors wortmannin and LY294002 were used before sevoflurane preconditioning to investigate the role of PI3K/Akt in the induction of ischaemic tolerance. (c) Graphical representation of the protocol for experiment III shows that CTMP and scramble lentivirus transduction were used 3 days before preconditioning to investigate the effect of over-expression of CTMP on sevoflurane-induced neuroprotection. Sevo, sevoflurane preconditioning; LV-CTMP, lentiviral vector Ubi-MCS-CTMP; LV-C, lentiviral vector-scramble. British Journal of Anaesthesia 2015 114, 327-335DOI: (10.1093/bja/aeu271) Copyright © 2015 The Author(s) Terms and Conditions

Fig 2 Effect of sevoflurane preconditioning on neurological scores and cerebral infarct volume at 1 and 7 days after reperfusion for I/R, PC+I/R (n=7). Sevoflurane preconditioning improved the neurological scores (a) and reduced the brain infarct volumes (b and c) of the rats after MCAO. Significance of experimental vs I/R animals is denoted by asterisk, *P<0.05, ** P<0.01. British Journal of Anaesthesia 2015 114, 327-335DOI: (10.1093/bja/aeu271) Copyright © 2015 The Author(s) Terms and Conditions

Fig 3 Effect of sevoflurane preconditioning on the expression of pSer473-Akt, pSer9 GSK-3β, and CTMP at 1, 3, 12, and 24 h after reperfusion (n=5, except I/R and PC+I/R at 24 h, n=4). Sevoflurane preconditioning suppressed hyperphosphorylation of Akt induced by focal cerebral ischaemia (a) and promoted phosphorylation of an Akt target GSK3β at Ser9 (b) at 1 and 3 h after reperfusion. Elevation of CTMP expression induced by focal cerebral ischaemia in penumbra tissues was inhibited by sevoflurane preconditioning at 3 and 12 h after reperfusion (c). Sevoflurane preconditioning increased Akt activity of penumbra tissues 3 h after reperfusion, whereas ischaemia did not (d). Significance of experimental vs sham animals is denoted by asterisk; PC+I/R vs I/R is denoted by hash. */#P<0.05, **/##P<0.01. British Journal of Anaesthesia 2015 114, 327-335DOI: (10.1093/bja/aeu271) Copyright © 2015 The Author(s) Terms and Conditions

Fig 4 Effect of the wortmannin and LY294002 on the neuroprotection of sevoflurane preconditioning (n=6 for VE-LY+PC+I/R; n=7 for I/R, PC+I/R and WT+I/R; n=8 for WT+PC+I/R, VE-WT+PC+I/R, and LY+PC+I/R). Wortmannin and LY294002 abolished the neuroprotective effects of sevoflurane preconditioning as demonstrated by cerebral infarct volume (b and c). There were no significant differences between the groups for neurological evaluation (a) and. Significance of experimental vs I/R animals is denoted by asterisk, **P<0.01. British Journal of Anaesthesia 2015 114, 327-335DOI: (10.1093/bja/aeu271) Copyright © 2015 The Author(s) Terms and Conditions

Fig 5 Expression of CTMP was up-regulated 72 h after lentivirus transduction (n=4, a), paralleling with a decreased Akt activity (n=4, b). Significance of experimental vs I/R animals is denoted by asterisk, **P<0.01. British Journal of Anaesthesia 2015 114, 327-335DOI: (10.1093/bja/aeu271) Copyright © 2015 The Author(s) Terms and Conditions

Fig 6 Over-expression of CTMP reversed the neuroprotective effects of sevoflurane preconditioning as demonstrated by cerebral infarct volume (b and c. n=6 for I/R and LV-C+I/R; n=7 for PC+I/R and LVCTMP+PC+I/R; n=8 for LV-C+PC+I/R and LV-CTMP+I/R). No significant differences were found in neurological scores (a). Significance of experimental vs I/R animals is denoted by asterisk; experimental vs PC+I/R animals is denoted by hash. #P<0.05, **/##P<0.01. British Journal of Anaesthesia 2015 114, 327-335DOI: (10.1093/bja/aeu271) Copyright © 2015 The Author(s) Terms and Conditions