Alterations of Glucosylceramide-β-Glucosidase Levels in the Skin of Patients with Psoriasis Vulgaris Francesca Alessandrini, Sabine Pfister, Elisabeth.

Slides:

Advertisements

Similar presentations

Lipodermatosclerosis is Characterized by Elevated Expression and Activation of Matrix Metalloproteinases: Implications for Venous Ulcer Formation Yared.

Advertisements

The CXC Receptor 2 Is Overexpressed in Psoriatic Epidermis

Persistence of Both Peripheral and Non-Peripheral Corneodesmosomes in the Upper Stratum Corneum of Winter Xerosis Skin Versus only Peripheral in Normal.

Ultraviolet Radiation Increases Tropoelastin mRNA Expression in the Epidermis of Human Skin In Vivo Jin Young Seo, Seong Hun Lee, Choon Shik Youn, Hai.

Proliferating Keratinocytes Are Putative Sources of the Psoriasis Susceptibility-Related EDA+(Extra Domain A of Fibronectin) Oncofetal Fibronectin Márta.

Topical Application of 17β-Estradiol Increases Extracellular Matrix Protein Synthesis by Stimulating TGF-β Signaling in Aged Human Skin In Vivo Eui Dong.

HAX-1, Identified by Differential Display Reverse Transcription Polymerase Chain Reaction, Is Overexpressed in Lesional Psoriasis Alireza Mirmohammadsadegh,

Peroxiredoxin is Ubiquitously Expressed in Rat Skin: Isotype-Specific Expression in the Epidermis and Hair Follicle Jeong Eun Lee, Byung Dae Kwon, Jee-Bum.

BM-40(Osteonectin, SPARC) Is Expressed Both in the Epidermal and in the Dermal Compartment of Adult Human Skin Nicholas Hunzelmann, Martin Hafner, Sabine.

Interleukin-17 and Interferon-γ Synergize in the Enhancement of Proinflammatory Cytokine Production by Human Keratinocytes Marcel B.M. Teunissen, Jan.

Skin Retinoid Concentrations Are Modulated by CYP26AI Expression Restricted to Basal Keratinocytes in Normal Human Skin and Differentiated 3D Skin Models

Elisabeth Riedl, Yayoi Tada, Mark C. Udey

Upregulation of Class II β-Tubulin Expression in Differentiating Keratinocytes Woong-Hee Lee, Joo-Young Kim, Young-Sik Kim, Hye-Joon Song, Ki-Joon Song,

Raija Tammi Journal of Investigative Dermatology

Type II Collagen Accumulation in Overlying Dermo-Epidermal Junction of Pilomatricoma Is Mediated by Bone Morphogenetic Protein 2 and 4 Hideki Mieno,

Molecular Cloning and Expression of Human Keratinocyte Proline-Rich Protein (hKPRP), an Epidermal Marker Isolated from Calcium-Induced Differentiating.

1α,25-Dihydroxyvitamin-D3-3-Bromoacetate Regulates AKT/mTOR Signaling Cascades: A Therapeutic Agent for Psoriasis Ananya Datta Mitra, Siba P. Raychaudhuri,

Analysis of Proteins with Caseinolytic Activity in a Human Stratum Corneum Extract Revealed a Yet Unidentified Cysteine Protease and Identified the So-Called.

Comparative Proteomic Profiling of Murine Skin

Degradation by Stratum Corneum Proteases Prevents Endogenous RNase Inhibitor from Blocking Antimicrobial Activities of RNase 5 and RNase 7 Arby Abtin,

Significance of the S100A4 Protein in Psoriasis

Yuangang Liu, James P. Lagowski, Shangpu Gao, James H

Protein C Inhibitor is Expressed in Keratinocytes of Human Skin

Cryptic Splicing at a Non-Consensus Splice-Donor in a Patient with a Novel Mutation in the Plakophilin-1 Gene Peter M. Steijlen, Maurice A.M. van Steensel,

Heat Modulation of Tropoelastin, Fibrillin-1, and Matrix Metalloproteinase-12 in Human Skin In Vivo Zhou Chen, Jin Young Seo, Yeon Kyung Kim, Se Rah.

Claus Schneider, W. David Strayhorn, Dana M. Brantley, Lillian B

Basal Keratinocytes from Uninvolved Psoriatic Skin Exhibit Accelerated Spreading and Focal Adhesion Kinase Responsiveness to Fibronectin Guofen Chen,

Sri Rajalakshmi Rudrabhatla, Christie L. Mahaffey, Mark E. Mummert

Volume 54, Issue 2, Pages (August 1998)

Characterization of the CC Chemokine Receptor 3 on Human Keratinocytes

Differential Expression of Phosphorylated NF-κB/RelA in Normal and Psoriatic Epidermis and Downregulation of NF-κB in Response to Treatment with Etanercept

Decreased Intraindividual HLA Class I Expression is due to Reduced Transcription in Advanced Melanoma and Does Not Correlate with HLA-G Expression Jörg.

Transcriptional Regulation of ATP2C1 Gene by Sp1 and YY1 and Reduced Function of its Promoter in Hailey–Hailey Disease Keratinocytes Hiroshi Kawada,

Noritaka Oyama, Keiji Iwatsuki, Yoshimi Homma, Fumio Kaneko

Interleukin-13 Receptor in Psoriatic Keratinocytes: Overexpression of the mRNA and Underexpression of the Protein Juan C. Cancino-Díaz, Elba Reyes-Maldonado,

The Paracrine Role of Stem Cell Factor/c-kit Signaling in the Activation of Human Melanocytes in Ultraviolet-B-Induced Pigmentation Akira Hachiya, Akemi.

The Melanocortin 5 Receptor is Expressed in Human Sebaceous Glands and Rat Preputial Cells Diane Thiboutot, Aruntha Sivarajah, Kathryn Gilliland, Zhaoyuan.

Denis Mehic, Latifa Bakiri, Minoo Ghannadan, Erwin F

The Epidermal Stem Cell Factor Is Over-Expressed in Lentigo Senilis: Implication for the Mechanism of Hyperpigmentation Hideko Hattori, Makoto Kawashima,

Human Keratinocytes Respond to Osmotic Stress by p38 Map Kinase Regulated Induction of HSP70 and HSP27 M. Garmyn, A. Pupe Journal of Investigative Dermatology

Keratinocytes Express the CD146 (Muc18/S-Endo) Antigen in Tissue Culture and During Inflammatory Skin Diseases1 Wolfgang Weninger, Michael Rendl, Michael.

Age-Related Alterations in the Inflammatory Response to Dermal Injury

Gereon Lauer, Stephan Sollberg, Melanie Cole, Thomas Krieg, Sabine A

Bikunin, a Serine Protease Inhibitor, is Present on the Cell Boundary of Epidermis Cui Chang-Yi, Yoshinori Aragane, Akira Maeda, Piao Yu-Lan, Masae Takahashi,

Expression of Interleukin-12 is Increased in Psoriatic Skin

Akemi Ishida-Yamamoto, Hidetoshi Takahashi, Hajime Iizuka

Rab3a and SNARE Proteins: Potential Regulators of Melanosome Movement

Scleroderma Fibroblasts Demonstrate Enhanced Activation of Akt (Protein Kinase B) In Situ Jae-Bum Jun, Melanie Kuechle, Junki Min, Seung Cheol Shim,

Multiple Epidermal Connexins are Expressed in Different Keratinocyte Subpopulations Including Connexin 31 Wei-Li Di, Elizabeth L. Rugg, Irene M. Leigh,

Expression of Perilipin A on the Surface of Lipid Droplets Increases along with the Differentiation of Hamster Sebocytes In Vivo and In Vitro Noriko.

I. Elisabeth Ekholm, Maria Brattsand, Torbjörn Egelrud

The Effect of Thioredoxin on the Expression of Proopiomelanocortin-Derived Peptides, the Melanocortin 1 Receptor and Cell Survival of Normal Human Keratinocytes

Overexpression of the Oncofetal Fn Variant Containing the EDA Splice-in Segment in the Dermal–Epidermal Junction of Psoriatic Uninvolved Skin Kathleen.

Kei Wada, Chihaya Maesawa, Toshihide Akasaka, Tomoyuki Masuda

Expression of Mast Cell Growth Modulating and Chemotactic Factors and their Receptors in Human Cutaneous Scars Barbara Hermes Journal of Investigative.

Research Snippets from the British Journal of Dermatology

Expression of FcRn, the MHC Class I-Related Receptor for IgG, in Human Keratinocytes Karla Cauza, Gabriele Hinterhuber, Ruth Dingelmaier-Hovorka, Karin.

Shigeru Kusuda, Cui Chang-Yi, Masae Takahashi, Tadashi Tezuka

Expression of Opsin Molecule in Cultured Murine Melanocyte

The Level of Prosaposin is Decreased in the Skin of Patients with Psoriasis Vulgaris Francesca Alessandrini, Silke Stachowitz, Johannes Ring, Heidrun.

Eric N. Johnson Journal of Investigative Dermatology

SLURP1 Is a Late Marker of Epidermal Differentiation and Is Absent in Mal de Meleda Bertrand Favre, Laure Plantard, Lorène Aeschbach, Noureddine Brakch,

David A. Norris, Brian L. Kotzin Journal of Investigative Dermatology

Permeability Barrier Disruption Increases the Level of Serine Palmitoyltransferase in Human Epidermis Francesca Alessandrini, Dr., Heidrun Behrendt

Journal of Investigative Dermatology

Kristiina Airola, Norbert E. Fusenig

Redistribution of LRIG Proteins in Psoriasis

Identification of a Commonly Used CDR3 Region of Infiltrating T Cells Expressing Vβ13 and Vβ15 Derived From Psoriasis Patients Ha Young Hwang, Young.

Effect of Healing on the Expression of Transforming Growth Factor βs and their Receptors in Chronic Venous Leg Ulcers Allison J. Cowin, Nicholas Hatzirodos,

Matrix Metalloproteinase Inhibitor BB-3103 Unlike the Serine Proteinase Inhibitor Aprotinin Abrogates Epidermal Healing of Human Skin Wounds Ex Vivo1

Presentation transcript:

Alterations of Glucosylceramide-β-Glucosidase Levels in the Skin of Patients with Psoriasis Vulgaris Francesca Alessandrini, Sabine Pfister, Elisabeth Kremmer, Josef-Karl Gerber, Johannes Ring, Heidrun Behrendt Journal of Investigative Dermatology Volume 123, Issue 6, Pages 1030-1036 (December 2004) DOI: 10.1111/j.0022-202X.2004.23469.x Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Gelelectrophoresis of GlcCer'ase RT-PCR products. Gelelectrophoresis of GlcCer'ase (GlcCer, upper lanes) and GAPDH (lower lanes) in representative normal epidermis (NS), and in five psoriatic subjects (P1–P5), non-lesional skin left (a), lesional skin right (b). Total cellular RNA was isolated from epidermal specimen (NS, P1–P5). RT-PCR was performed with GlcCer'ase and GAPDH primer, and separated by gel electrophoresis on a 2% agarose gel stained with ethidium bromide as described (see Materials and Methods) Journal of Investigative Dermatology 2004 123, 1030-1036DOI: (10.1111/j.0022-202X.2004.23469.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Semiquantitative analysis of GlcCer'ase PCR bands and statistics. Relative density levels of GlcCer'ase PCR bands are expressed in percentage of GAPDH. The graph in (a) shows control levels in a representative sample of normal skin (NS) and the levels in non-lesional (left) and lesional (right) skin of five psoriatic subjects (P1–P5). The densitometric values are obtained from the corresponding ethidiumbromide-stained PCR reaction products presented in Figure 1. The box plot in (b) shows the mean, SE and SD of the values in non-lesional (NL) and lesional (L) skin of psoriatic subjects (n=5), *p<0.05 Journal of Investigative Dermatology 2004 123, 1030-1036DOI: (10.1111/j.0022-202X.2004.23469.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Western Immunoblotting of human monoclonal anti-GBC antibody. Aliquots of human Cerezyme (1) and GBC purified protein (2) were separated by SDS-PAGE, blotted with monoclonal anti-GBC antibody and developed (see Materials and Methods) Journal of Investigative Dermatology 2004 123, 1030-1036DOI: (10.1111/j.0022-202X.2004.23469.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Immunohistochemical localization of GlcCer'ase is showing in normal control skin (a), in non-lesional (c) and lesional (d) skin of psoriatic subjects and non-specific primary negative control (b). The series in (c) and (d) represents one individual. The immunohistochemical analysis was performed using the ABC method (see Materials and Methods). GlcCer'ase protein in normal control skin was localized in the granular–corneocyte transition zone and in the stratum corneum (a, arrows). In psoriatic non-lesional skin (c) GlcCer'ase immunolocalization was decreased compared to control and was present as spotty reaction in the granular–corneocyte transition zone (arrow). In psoriatic lesional skin (d) GlcCer'ase was increased compared with (c) in the upper stratum granulosum and in the lower stratum corneum (arrows). No staining was detected using a non-specific primary antibody (b). Scale bar=30 μm Journal of Investigative Dermatology 2004 123, 1030-1036DOI: (10.1111/j.0022-202X.2004.23469.x) Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions