Methylation dependency of SNYCRIP protein interactions.

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Methylation dependency of SNYCRIP protein interactions. Methylation dependency of SNYCRIP protein interactions. (A) Structure of SMN1 Tudor domain monomer and binding mutant used in this study (Tripsianes, 2011, PDB 4AEA). (B) Structure of PHF Tudor domain and binding mutant used in this study (Musselman, 2012, PDB 4HCZ). (C) SMN1 dimerization LUMIER experiment. LUMIER experiment testing SMN1 and PHF1 mutants against WT SMN1. Experiments undertaken in triplicate, with error bars representing highest and lowest observed values. Mann–Whitney–Wilcoxon test used to determine statistical significance. *P < 0.05. TD, Tudor domain; DD, dimerization domain. Jonathan Woodsmith et al. LSA 2018;1:e201800178 © 2018 Stelzl et al.