AMRs in the turnover of secreted proteins.

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AMRs in the turnover of secreted proteins. AMRs in the turnover of secreted proteins. (A) Plasma proteins from mice lacking Asgr1 or Asgr2 were isolated by lectin affinity chromatography and analyzed by SDS/PAGE and lectin blotting (Top) among amounts of protein indicated by silver staining (Bottom). (B) Quantification of lectin ligands detected among plasma proteins in A normalized to results from WT littermates. (C) Abundance of Neu1, Neu2, Neu3, or Neu4 proteins in plasma. (D) Plasma Neu activity. (E) Plasma TNAP and IAP activities measured in immune precipitates normalized to WT littermates. (F) Abundance of TNAP and IAP proteins in plasma measured by SDS/PAGE and immunoblotting. (G and H) Biotinylated in vivo t1/2 analyses of TNAP and IAP. (I and J) Lectin binding to equivalent amounts of immunoprecipitated TNAP and IAP. Data are representative of six to eight separate littermate cohorts of the indicated genotypes, and are presented as means ± SEM (***P < 0.001; **P < 0.01; *P < 0.05). Won Ho Yang et al. PNAS 2015;112:44:13657-13662 ©2015 by National Academy of Sciences