Ectopic overexpression of WOR3 drives switching to the opaque cell type. Ectopic overexpression of WOR3 drives switching to the opaque cell type. (A) Visualization.

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Ectopic overexpression of WOR3 drives switching to the opaque cell type. Ectopic overexpression of WOR3 drives switching to the opaque cell type. (A) Visualization of Wor3–GFP fusion protein in opaque cells. From left to right, merged image, differential interference contrast microscopy (DIC), fluorescence of GFP, staining by DAPI. (B) Ectopic overexpression of WOR3 in white cells at 25 °C results in mass switching to the opaque cell type. (C) Wild-type and wor3 deletion white and opaque colonies. (D) Wild-type and wor3 deletion white and opaque cells. (E) Opaque sectors, indicated with lines, are formed by a white colony derived from a wor3 deletion strain. Matthew B. Lohse et al. PNAS 2013;110:19:7660-7665 ©2013 by National Academy of Sciences