Volume 14, Issue 6, Pages (June 2007)

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Volume 14, Issue 6, Pages 703-714 (June 2007) Insights into Polyether Biosynthesis from Analysis of the Nigericin Biosynthetic Gene Cluster in Streptomyces sp. DSM4137 Barbara M. Harvey, Tatiana Mironenko, Yuhui Sun, Hui Hong, Zixin Deng, Peter F. Leadlay, Kira J. Weissman, Stephen F. Haydock Chemistry & Biology Volume 14, Issue 6, Pages 703-714 (June 2007) DOI: 10.1016/j.chembiol.2007.05.011 Copyright © 2007 Elsevier Ltd Terms and Conditions

Figure 1 Structures of the Polyethers Nigericin, Abierixin, Monensin, and Nanchangmycin Chemistry & Biology 2007 14, 703-714DOI: (10.1016/j.chembiol.2007.05.011) Copyright © 2007 Elsevier Ltd Terms and Conditions

Figure 2 The Organization of the Nigericin Biosynthetic Gene Cluster The proposed functions of the genes shown are indicated by their color; modular PKS genes are shown in green, auxiliary PKS genes are shown in brown, the SAM-dependent methyltranferase is shown in pink, the thioesterase is shown in orange, the cytochrome P450 hydroxylase is shown in blue, the FAD-dependent epoxidase is shown in yellow, the cyclases are shown in red, and a gene encoding a hypothetical protein is shown in gray. Chemistry & Biology 2007 14, 703-714DOI: (10.1016/j.chembiol.2007.05.011) Copyright © 2007 Elsevier Ltd Terms and Conditions

Figure 3 LC-MS Analysis of Nigericin-Related Metabolites Produced by Mutant Strains of Streptomyces sp. DSM4137 (A) LC-MS chromatogram of the sodiated ion (m/z 747.5) from nigericin standard. (B) LC-MS chromatogram of the sodiated ion (m/z 747.5) from the azalomycin- and meridamycin-deleted strain. (C) LC-MS chromatogram of the sodiated ion (m/z 747.5) from the azalomycin-, meridamycin-, and nigericin-deleted strain. Chemistry & Biology 2007 14, 703-714DOI: (10.1016/j.chembiol.2007.05.011) Copyright © 2007 Elsevier Ltd Terms and Conditions

Figure 4 A Comparison of the Organization of the First Nine PKS Modules of Nigericin, Monensin, and Nanchangmycin Biosynthesis Inactive domains are shaded gray and are named in italics, AT domains specific for malonate extender units are shown in pink, and AT domains specific for methylmalonate extender units are shown in blue. Chemistry & Biology 2007 14, 703-714DOI: (10.1016/j.chembiol.2007.05.011) Copyright © 2007 Elsevier Ltd Terms and Conditions

Figure 5 Docking Domains (A) Multiple alignment of C-terminal docking domains from modular PKSs demonstrates that the docking domains from NigA9 and MonA8 fall into the sequence group with DEBS 2. In contrast, the sequence of the C-terminal docking domain from NanA11 belongs within a putative second group of C-terminal docking domains. The extent of helix 3 (bar) along with the hydrophobic residues present in the docking interface (arrows) are indicated. (B) Multiple alignment of the N-terminal docking domain partners of the C termini shown in Figure 4A. MonKSX and NigKSX fit into the sequence group with DEBS 3, while NanA9 clusters with the second group of N-terminal docking domains. The extent of the coiled-coil region (bar) as well as the hydrophobic interface residues (arrows) are indicated. PIK, pikromycin; NYS, nystatin; RAPS, rapamycin; TYL, tylosin; RIF, rifamycin; OLEA; oleandomycin; DEBS, erythromycin. Chemistry & Biology 2007 14, 703-714DOI: (10.1016/j.chembiol.2007.05.011) Copyright © 2007 Elsevier Ltd Terms and Conditions