Christopher J. Blewett, MD, Robert E. Cilley, MD, H

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Presentation transcript:

Regenerative healing of incisional wounds in midgestational murine hearts in organ culture Christopher J. Blewett, MD, Robert E. Cilley, MD, H.Paul Ehrlich, PhD, James H. Blackburn, MD, Peter W. Dillon, MD, Thomas M. Krummel, MD The Journal of Thoracic and Cardiovascular Surgery Volume 113, Issue 5, Pages 880-885 (May 1997) DOI: 10.1016/S0022-5223(97)70260-5 Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 1 Photomicrographs of organ cultured hearts. A, Wounded 14-day heart after 2 days in culture stained with hematoxylin and eosin. Arrows identify the wound site, which is otherwise difficult to distinguish from unwounded tissue. B, Cultured 18-day heart harvested 7 days after wounding, stained with trichrome stain. Tissue repair is absent, as demonstrated by the open space between the arrows. C, A wounded 22-day heart explant (wound located between the arrows), which was maintained for 7 days after wounding, stained with trichrome stain. The transition to adult-type healing with scar formation has occurred (as shown in the insert), where newly deposited, poorly organized granulation tissue is deposited. The Journal of Thoracic and Cardiovascular Surgery 1997 113, 880-885DOI: (10.1016/S0022-5223(97)70260-5) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 1 Photomicrographs of organ cultured hearts. A, Wounded 14-day heart after 2 days in culture stained with hematoxylin and eosin. Arrows identify the wound site, which is otherwise difficult to distinguish from unwounded tissue. B, Cultured 18-day heart harvested 7 days after wounding, stained with trichrome stain. Tissue repair is absent, as demonstrated by the open space between the arrows. C, A wounded 22-day heart explant (wound located between the arrows), which was maintained for 7 days after wounding, stained with trichrome stain. The transition to adult-type healing with scar formation has occurred (as shown in the insert), where newly deposited, poorly organized granulation tissue is deposited. The Journal of Thoracic and Cardiovascular Surgery 1997 113, 880-885DOI: (10.1016/S0022-5223(97)70260-5) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 1 Photomicrographs of organ cultured hearts. A, Wounded 14-day heart after 2 days in culture stained with hematoxylin and eosin. Arrows identify the wound site, which is otherwise difficult to distinguish from unwounded tissue. B, Cultured 18-day heart harvested 7 days after wounding, stained with trichrome stain. Tissue repair is absent, as demonstrated by the open space between the arrows. C, A wounded 22-day heart explant (wound located between the arrows), which was maintained for 7 days after wounding, stained with trichrome stain. The transition to adult-type healing with scar formation has occurred (as shown in the insert), where newly deposited, poorly organized granulation tissue is deposited. The Journal of Thoracic and Cardiovascular Surgery 1997 113, 880-885DOI: (10.1016/S0022-5223(97)70260-5) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 1 Photomicrographs of organ cultured hearts. A, Wounded 14-day heart after 2 days in culture stained with hematoxylin and eosin. Arrows identify the wound site, which is otherwise difficult to distinguish from unwounded tissue. B, Cultured 18-day heart harvested 7 days after wounding, stained with trichrome stain. Tissue repair is absent, as demonstrated by the open space between the arrows. C, A wounded 22-day heart explant (wound located between the arrows), which was maintained for 7 days after wounding, stained with trichrome stain. The transition to adult-type healing with scar formation has occurred (as shown in the insert), where newly deposited, poorly organized granulation tissue is deposited. The Journal of Thoracic and Cardiovascular Surgery 1997 113, 880-885DOI: (10.1016/S0022-5223(97)70260-5) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 2 Outgrowth of cells from murine heart explants. A 14-day murine heart explant showing cell outgrowth from the edge of the explant in the direction of the arrow. Note the distance the cells migrated and the circumference of the explant edge involved in outgrowth. The Journal of Thoracic and Cardiovascular Surgery 1997 113, 880-885DOI: (10.1016/S0022-5223(97)70260-5) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 3 Actin filament fluorescent staining of 4-day murine heart explant outgrowth. A, A 14-day heart explant showing an intact sheet of cells growing from the edge of the explant with phalloidin-stained actin stress fibers at the periphery of the cells. B, A monolayer of cells growing out from an 18-day murine heart explant showing more randomly arranged cells having actin stress fibers running in parallel arrays along the long axis of the cell. (Original magnifications ×120.) The Journal of Thoracic and Cardiovascular Surgery 1997 113, 880-885DOI: (10.1016/S0022-5223(97)70260-5) Copyright © 1997 Mosby, Inc. Terms and Conditions

Fig. 3 Actin filament fluorescent staining of 4-day murine heart explant outgrowth. A, A 14-day heart explant showing an intact sheet of cells growing from the edge of the explant with phalloidin-stained actin stress fibers at the periphery of the cells. B, A monolayer of cells growing out from an 18-day murine heart explant showing more randomly arranged cells having actin stress fibers running in parallel arrays along the long axis of the cell. (Original magnifications ×120.) The Journal of Thoracic and Cardiovascular Surgery 1997 113, 880-885DOI: (10.1016/S0022-5223(97)70260-5) Copyright © 1997 Mosby, Inc. Terms and Conditions