Zone of inhibition assay.

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Kirby-Bauer disk diffusion Testing skill based learning

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Zone of inhibition assay. Zone of inhibition assay. R. sphaeroides strains were cultivated under microaerobic conditions at 32°C in biological triplicates. 200 μl culture with an OD660 of 0.4 was mixed with prewarmed top agar (0.8% agar) and layered onto malate minimal salt medium plates. When top agar was solidified, a filter disk containing 5 μl of the oxidative stress agent was placed in the center of the plate. Concentration of the agents used are 1 M H2O2, 0.5 M tBOOH, 0.5 M paraquat, and 10 mM methylene blue (MB). Values of the diameter (millimeter) of the zone of growth inhibition are displayed in the diagram. The given values are the means of the technical duplicates of three independent biological replicates. Konrad U Förstner et al. LSA 2018;1:e201800080 © 2018 Förstner et al.