Adenosine-induced osteogenic differentiation of hiPSCs involves A2bR

Slides:

Advertisements

Similar presentations

High molecular weight hyaluronic acid regulates osteoclast formation by inhibiting receptor activator of NF-κB ligand through Rho kinase W. Ariyoshi,

Advertisements

ORL1 inhibits axon regeneration.

Volume 3, Issue 1, Pages 5-14 (July 2014)

Z. Zhang, Y. Kang, Z. Zhang, H. Zhang, X. Duan, J. Liu, X. Li, W. Liao

AβOs bind to and impact hypothalamic neurons Representative immunocytochemistry images of mature hypothalamic neurons in culture exposed to vehicle (Veh)

Fig. 3. Cytokines trigger proliferation and block differentiation of perivascular adipocytes. Cytokines trigger proliferation and block differentiation.

Fig. 5. Circulating PPi concentration does not correlate with severity of calcification phenotype in mice. Circulating PPi concentration does not correlate.

Volume 5, Issue 6, Pages (December 2015)

In vivo function of MeTro sealants using rat incision model of lungs

Fig. 1. BCAS1 expression identifies newly generated oligodendrocytes.

Fig. 3. Obese IFN-γ−/− mice develop accelerated NAFLD with fibrosis.

Molecular Therapy - Methods & Clinical Development

CaMKII inhibition in human primary and pluripotent stem cell-derived chondrocytes modulates effects of TGFβ and BMP through SMAD signaling B. Saitta,

PVSRIPO-mediated APC activation occurs in immunosuppressive conditions

Fig. 4 Infection-induced CLV dysfunction is associated with decreased LMC coverage. Infection-induced CLV dysfunction is associated with decreased LMC.

Volume 29, Issue 4, Pages (May 2014)

Fig. 5. Stimulation of EPO expression and secretion under hypoxic culture conditions. Stimulation of EPO expression and secretion under hypoxic culture.

Immunofluorescence staining of virus-infected human placental explants

Fig. 7 Gel scaffold for inhibition of postsurgical recurrence of B16F10 tumors. Gel scaffold for inhibition of postsurgical recurrence of B16F10 tumors.

Disrupted dynamics of early stages of differentiation perturbs neural cell fate specification. Disrupted dynamics of early stages of differentiation perturbs.

Growth characteristics of strain 22A and its MDH mutant derivatives.

Fig. 4 The E57K mutation in Fbln4 has differential effects on elastic versus resistance artery integrity. The E57K mutation in Fbln4 has differential effects.

Development and Dynamic Regulation of Mitochondrial Network in Human Midbrain Dopaminergic Neurons Differentiated from iPSCs Du Fang, Yu Qing, Shijun.

Endochondral bone formation in HLA-B27 transduced ATDC5 micromasses.

Primary cilia disassembly down-regulates mechanosensitive hedgehog signalling: a feedback mechanism controlling ADAMTS-5 expression in chondrocytes C.L.

AG-221 can reduce intracellular 2HG levels and induce differentiation in primary human IDH2R140Q- or IDH2R172K-mutant AML patient samples treated ex vivo.

Volume 4, Issue 6, Pages (June 2015)

Fig. 2. Binding of recombinant full-length Lm-411 to muscle receptors, myotubes, and self-polymerization is enhanced by mag and αLNNd. Binding of recombinant.

Fig. 5 Local gel scaffold for T cell memory response.

Fig. 7 CSPG4-high GBMs show more microglia than CSPG4-low GBMs and express TNFα. CSPG4-high GBMs show more microglia than CSPG4-low GBMs and express TNFα.

Fig. 5. Nutlin-3 treatment rescues the proliferation and differentiation of NPCs in vitro. Nutlin-3 treatment rescues the proliferation and differentiation.

Fig. 1. β-APP overexpression or exposure to inflammatory mediators induces sIBM-like pathology in cultured rat myocytes that is abrogated by arimoclomol.

DFP-induced astrogliosis is reduced by urethane.

Fig. 6 Photoreceptor cell survival in the RCS rat retina after transplantation with hESC-RPE cell sheets. Photoreceptor cell survival in the RCS rat retina.

Development and Dynamic Regulation of Mitochondrial Network in Human Midbrain Dopaminergic Neurons Differentiated from iPSCs Du Fang, Yu Qing, Shijun.

GDF11 induces myotube atrophy in vitro

The SI-Induced Increase in PA Levels Is Mediated by PbrPLDδ1 in Pollen

Fig. 1 Device structure, typical output performance, and cytocompatibility of BD-TENG. Device structure, typical output performance, and cytocompatibility.

Osteogenesis in monolayer HLA-B27 transduced hPDCs.

Fig. 4. Seeding density modulates cell shape in both cell types

Fig. 2. Proportion of motile objects and track length

Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate. Inflammation is associated with increased basal-cell plasticity.

Fig. 2. Ex vivo inducible knockout of PDCD2 in ESCs results in loss of S phase entry and increased p53.(A) Growth curve of inducible knockout and WT ESCs.

Fig. 4 hiPSC-derived osteoblasts (Ad-hiPSCs) contribute to the healing of critical-sized bone defects through the formation of vascularized neobone tissue.

Fig. 2 AdOPG transduction changes RANKL/OPG homeostasis in primary hMSCs differentiated on Col-GAG and MC-GAG. AdOPG transduction changes RANKL/OPG homeostasis.

Volume 12, Issue 1, Pages (January 2019)

Loss of LAR phosphatase activity is associated with decreased adhesion complex formation. Loss of LAR phosphatase activity is associated with decreased.

Fig. 1. Phenotypic characterisation of primary human tubular epithelial cells and human renal fibroblasts. Phenotypic characterisation of primary human.

Fgfr3;4 mutant lungs display an increase in Mfap5, Igf1 and Fbn2 expression. Fgfr3;4 mutant lungs display an increase in Mfap5,Igf1and Fbn2 expression.

Fig. 2. iPSCs produce functional osteoblasts.

ALS-CSF induces atrophy of type 2 fibres in EDL muscle of neonatal rats. ALS-CSF induces atrophy of type 2 fibres in EDL muscle of neonatal rats. (A,B)

Fig. 4. Reduction of apoptosis and autophagic stress by treatment with silibinin in the KA-treated hippocampus. (A, B) Double-immunofluorescence staining.

CPPED1 (A) and PPARγ2 (B) mRNA expressions in cultured SGBS cells during adipocyte differentiation. CPPED1 (A) and PPARγ2 (B) mRNA expressions in cultured.

P.J. Moss, W. Huang, J. Dawes, K. Okuse, S.B. McMahon, A.S.C. Rice

Fig. 5. Vitamin B12 supplementation in the host altered the transcriptome of P. acnes in the skin microbiota. Vitamin B12 supplementation in the host altered.

Ctip2 directly regulates Notch1 expression in differentiated cells.

Fig. 1. DEL-1 is expressed by human and mouse osteoclasts.

Fig. 6 Activating IRF8 promotes macrophage migration during the recovery phase after SCI. Activating IRF8 promotes macrophage migration during the recovery.

Volume 8, Issue 6, Pages (June 2017)

Fig. 3 IRF8 is required for macrophage migration toward the epicenter in the injured spinal cord during the recovery phase. IRF8 is required for macrophage.

Fig. 1 Deficient CD73 and CD39 expressions and extracellular adenosine concentration in BM of OVX animals. Deficient CD73 and CD39 expressions and extracellular.

Functional assessment of NF-κB activation in SKBR3 cells.

Fig. 4 Effects of morphogen priming of engineered mesenchymal condensations on in vitro chondrogenic lineage specification at the time of implantation.

Fig. 4 Adenosine A2BR signaling promote osteogenic and inhibit osteoclast differentiation in vitro. Adenosine A2BR signaling promote osteogenic and inhibit.

Lineage-specific decline of E2F1 nuclear levels during oligodendrocyte differentiation. Lineage-specific decline of E2F1 nuclear levels during oligodendrocyte.

Fig. 5 C9orf72 knockdown disrupts autophagy induction.

IFN treatment of human midgestation villous explants induces syncytial knot formation. IFN treatment of human midgestation villous explants induces syncytial.

Fig. 5 Reconstitution of the dormant state using fetal ovaries.

related to Fig 1. Isolation and characterization of FAPs.

Fig. 4 Systemic AAV9 delivery of gene editing components to ΔEx44 mice rescues dystrophin expression. Systemic AAV9 delivery of gene editing components.

Presentation transcript:

Fig. 2 Adenosine-induced osteogenic differentiation of hiPSCs involves A2bR. Adenosine-induced osteogenic differentiation of hiPSCs involves A2bR. (A) Time-dependent quantitative gene expressions of hiPSCs for adenosine receptor subtypes (A1R, A2aR, A2bR, and A3R) cultured in growth medium (GM), adenosine-supplemented growth medium (Adenosine), and osteogenic induction medium (OM). (B) Quantitative gene expression analysis of osteogenic markers (RUNX2, OCN, and SPP1) and pluripotent marker (NANOG) for hiPSCs after 21 days of culture. The plus (+) and minus (−) symbols in gene expression denote growth medium in the presence and absence of adenosine or PSB 603 (an A2bR antagonist). (C) Immunofluorescence staining for osteocalcin (green), F-actin (red), and nuclei (blue), as well as Alizarin Red S staining of hiPSCs cultured for 21 days in the presence and absence of adenosine or PSB 603. Inset shows the stained image of the whole well. Various media include growth medium (GM), growth medium containing adenosine (Adenosine), and growth medium containing both adenosine and PSB 603 (Adenosine + PSB 603). Scale bars, 100 μm. Data are shown as means ± SEs (n = 3). Data are presented as fold expression of target genes after normalization to undifferentiated, pluripotent hiPSCs. For A1R, A2aR, A2bR, and A3R, as well as RUNX2, OCN, and SPP1, the groups with various medium conditions at the same culture time were compared by one-way ANOVA with Tukey-Kramer post hoc test. For NANOG, all the groups were compared to undifferentiated, pluripotent hiPSCs by two-way ANOVA with Bonferroni post hoc test. Asterisks indicate statistical significances according to P values (*P < 0.05; **P < 0.01; ***P < 0.001). Heemin Kang et al. Sci Adv 2016;2:e1600691 Published by AAAS