Dynamic ranges of anti-PnPS IgG Reference standard serum 007sp for each of 13 serotypes. Dynamic ranges of anti-PnPS IgG Reference standard serum 007sp for each of 13 serotypes. MFI signal data for the reference standard curve are on the y axis, and specific IgG antibody concentration data are on the x axis. The blue line indicates the assay range lower limit as determined in the assay validation; the red line indicates the assay range upper limit. Danka Pavliakova et al. mSphere 2018; doi:10.1128/mSphere.00128-18