Scale-up of Theorem Proving

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Presentation transcript:

Scale-up of Theorem Proving MEC Meeting Hanyang University Proteome Research Lab 2002. 11. 29 Park, Ji-Yoon

Contents Primer Design Thermodynamics New Amplification and Detection Loop-mediated Isothermal Amplification(LAMP) Ramplification Amplification Method(RAM) Rolling Circle Amplification(RCA) Structure Specific Enzyme Cleavage

The Primer Design

Nucleic Acid Thermodynamic Parameters and Structural Motifs

Ramplification Amplification Methods

Loop-Mediated Isothermal Amplification Nucleic Acids Res. 28, e63(2000) 13

Invasive Cleavage Reaction Nat. Biotechnol. 19, 1053-1059(2001) Directly detect genomic DNA No target amplification required Sensitive & Accurate Easy to integrate into the routine laboratory

In Theorem Proving… Non-PCR Amplification Effective Detection Consideration of several parameter