PhenoGraph-based visualization on tSNE plots.

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PhenoGraph-based visualization on tSNE plots. PhenoGraph-based visualization on tSNE plots. LLC-NT or LLC-sh21 cells were orthotopically injected into the left lung lobes of mice and established primary tumors. After 2 wk of tumor growth with no treatment, the mice were euthanized and their tumor-bearing lung lobes were isolated. Single-cell suspensions were made from tumor-bearing lung lobes or naive lungs. Naive biological replicates each contained lungs isolated from one mouse (three mice/three replicates total). LLC-NT and LLC-sh21 biological replicates each contained tumor-bearing lungs from three pooled mice (nine mice/three replicates total; three mice/replicate). Single-cell suspensions were then stained with a 39-antibody panel and analyzed on the Helios mass cytometer. Data show all viable single cells, subjected to the PhenoGraph algorithm. (A) PhenoGraph-based visualization on a tSNE plot, colored according to the expression of the cellular markers (parameters) not included in Fig S5: INOS, Tim3, CXCR3, IkBA, Arg1, NKp64, Ki67, CD103, PD-L1, IRF4, Foxp3, CD80/86, B-catenin, CD127, GITR, CD25, p-H2AX, CTLA-4, PD-1, Ly6A/E, CD117, Lag3, and ICOS. Bonnie L Bullock et al. LSA 2019;2:e201900328 © 2019 Bullock et al.