Direct visualization by RNA FISH of TLR7 escape from X inactivation in B cells from women. Direct visualization by RNA FISH of TLR7 escape from X inactivation.

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Direct visualization by RNA FISH of TLR7 escape from X inactivation in B cells from women. Direct visualization by RNA FISH of TLR7 escape from X inactivation in B cells from women. RNA FISH was performed on CD19+ CD27+ B cells from a woman donor after 2 hours of in vitro stimulation with IFN-β. The images show two confocal planes from a Z-stack after hybridization with fluorescent probes for TLR7 (red) and XIST (green) transcripts. Fluorescent TLR7-specific probes were generated from a collection of PCR amplimers mapping to nonrepetitive regions and covering 7322 base pairs of the TLR7 gene. Arrows denote TLR7 transcripts (red) from the active [XIST RNA-negative (Xa)] and the inactive [XIST RNA-positive (Xi); green] X chromosome on two different confocal planes. Boxed areas are shown as enlarged images (right). Nuclei are counterstained with DAPI. Mélanie Souyris et al. Sci. Immunol. 2018;3:eaap8855 Copyright © 2018 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works